ArticleJournal of experimental botany2015
Measuring the mechanical properties of plant cells by combining micro-indentation with osmotic treatments.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Synthetic pectin-cellulose nanofiber capsule recapitulates the mechanical properties of a regenerating plant cell wall.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Mechanical interactions between tissue layers underlie plant morphogenesis.Nature plants · 2025Article
- The viscoelastic properties of Nicotiana tabacum BY-2 suspension cell lines adapted to high osmolarity.BMC plant biology · 2025Article
- Arabidopsis floral buds are locked through stress-induced sepal tip curving.Nature plants · 2024Article
- A mechanohydraulic model supports a role for plasmodesmata in cotton fiber elongation.PNAS nexus · 2024Article
- Hidden functional complexity in the flora of an early land ecosystem.The New phytologist · 2024Article
- Cell wall dynamics: novel tools and research questions.Journal of experimental botany · 2023Review
- Review
- Elastic shell theory for plant cell wall stiffness reveals contributions of cell wall elasticity and turgor pressure in AFM measurement.Scientific reports · 2022Article
- Protocol for mapping the variability in cell wall mechanical bending behavior in living leaf pavement cells.Plant physiology · 2022Article
- Plant cell mechanobiology: Greater than the sum of its parts.The Plant cell · 2022Review
- Cell biology of the leaf epidermis: Fate specification, morphogenesis, and coordination.The Plant cell · 2022Review
- Cell wall and cytoskeletal contributions in single cell biomechanics ofQuantitative plant biology · 2022Article
- Between Stress and Response: Function and Localization of Mechanosensitive CaInternational journal of molecular sciences · 2021Review
- PECTATE LYASE LIKE12 patterns the guard cell wall to coordinate turgor pressure and wall mechanics for proper stomatal function in Arabidopsis.The Plant cell · 2021Article
- 3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy.Nature communications · 2021Article
- A Life Cycle for Modeling Biology at Different Scales.Frontiers in plant science · 2021Review
- Structure and Biomechanics during Xylem Vessel Transdifferentiation inPlants (Basel, Switzerland) · 2020Article
- Pectin methylesterase selectively softens the onion epidermal wall yet reduces acid-induced creep.Journal of experimental botany · 2020Article
- Force microscopy of theMicrosystems & nanoengineering · 2020Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Growth in plants results from the interaction between genetic and signalling networks and the mechanical properties of cells and tissues. There has been a recent resurgence in research directed at understanding the mechanical aspects of growth, and their feedback on genetic regulation. This has been driven in part by the development of new micro-indentation techniques to measure the mechanical properties of plant cells in vivo. However, the interpretation of indentation experiments remains a challenge, since the force measures results from a combination of turgor pressure, cell wall stiffness, and cell and indenter geometry. In order to interpret the measurements, an accurate mechanical model of the experiment is required. Here, we used a plant cell system with a simple geometry, Nicotiana tabacum Bright Yellow-2 (BY-2) cells, to examine the sensitivity of micro-indentation to a variety of mechanical and experimental parameters. Using a finite-element mechanical model, we found that, for indentations of a few microns on turgid cells, the measurements were mostly sensitive to turgor pressure and the radius of the cell, and not to the exact indenter shape or elastic properties of the cell wall. By complementing indentation experiments with osmotic experiments to measure the elastic strain in turgid cells, we could fit the model to both turgor pressure and cell wall elasticity. This allowed us to interpret apparent stiffness values in terms of meaningful physical parameters that are relevant for morphogenesis.
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