ArticleJournal of experimental botany2015
The dominant negative ARM domain uncovers multiple functions of PUB13 in Arabidopsis immunity, flowering, and senescence.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 96 citations in OpenAlex.
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- The ubiquitin code of receptor kinases in plants.Journal of integrative plant biology · 2026Review
- The ABAP1 interacting protein 10 (AIP10) exerts a dual role in the cell cycle and primary metabolism pathways in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2025Article
- TheFrontiers in plant science · 2025Article
- SARM1: a key multifaceted component in immunoregulation, inflammation and neurodegeneration.Frontiers in immunology · 2025Review
- Article
- Genome-wide analysis of the U-box E3 ligases gene family in potato (Solanum tuberosum L.) and overexpress StPUB25 enhance drought tolerance in transgenic Arabidopsis.BMC genomics · 2024Article
- Genome wide association study reveals new genes for resistance to striped stem borer in rice (Frontiers in plant science · 2024Article
- Rose long noncoding RNA lncWD83 promotes flowering by modulating ubiquitination of the floral repressor RcMYC2L.Plant physiology · 2023Article
- U-box E3 ubiquitin ligase PUB8 attenuates abscisic acid responses during early seedling growth.Plant physiology · 2023Article
- An activated form of NB-ARC protein RLS1 functions with cysteine-rich receptor-like protein RMC to trigger cell death in rice.Plant communications · 2023Article
- Genetic control of flowering time and fruit yield in citron watermelon.Frontiers in plant science · 2023Article
- Emerging roles of the ubiquitin-proteasome pathway in enhancing crop yield by optimizing seed agronomic traits.Plant cell reports · 2022Review
- Cooperative regulation of PBI1 and MAPKs controls WRKY45 transcription factor in rice immunity.Nature communications · 2022Article
- Ubiquitination of Receptorsomes, Frontline of Plant Immunity.International journal of molecular sciences · 2022Review
- How Many Faces Does the Plant U-Box E3 Ligase Have?International journal of molecular sciences · 2022Review
- Apple U-box-type E3 ubiquitin ligase MdPUB23 reduces cold-stress tolerance by degrading the cold-stress regulatory protein MdICE1.Horticulture research · 2022Article
- Identification and expression analysis ofPlant signaling & behavior · 2021Article
- Understanding and Exploiting Post-Translational Modifications for Plant Disease Resistance.Biomolecules · 2021Review
- The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2.Horticulture research · 2021Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Regulating the intensity and duration of immune responses is crucial to combat infections without deleterious side effects. Arabidopsis FLS2, the receptor for bacterial flagellin, activates immune signalling by association with its partner BAK1. Upon flagellin (flg22) perception, the plant U-box E3 ubiquitin ligases PUB12 and PUB13 complex with FLS2 in a BAK1-dependent manner, and ubiquitinate FLS2 for protein degradation, thereby down-regulating flagellin signalling. Domain deletion analysis indicates that the ARM domain of PUB13 interacts with the FLS2-BAK1 complex and is phosphorylated by BAK1. Overexpression of the PUB13 ARM domain alone inhibits flg22-induced FLS2-PUB13 association and PUB12/13-mediated FLS2 ubiquitination and degradation in Arabidopsis, suggesting that ectopic expression of the ARM domain in planta generates a dominant negative effect via blocking the ubiquitination activity. Similar to the pub12pub13 double mutant, transgenic plants expressing the PUB13 ARM domain display enhanced immune responses compared with wild-type plants. Moreover, PUB13ARM transgenic plants and the pub12pub13 mutant are more sensitive to stress-induced leaf senescence accompanied by elevated expression of stress-induced senescence marker genes. The resemblance between PUB13ARM transgenic plants and the pub12pub13 mutant provides genetic evidence that ectopic expression of the PUB ARM domain serves as an alternative approach to dissect the overlapping functions of closely related PUB genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.