ArticleLasers in surgery and medicine2015
Comparing high-resolution microscopy techniques for potential intraoperative use in guiding low-grade glioma resections.
Article in Lasers in surgery and medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Analysis of the Porphyrin Peak Shift and Fluorescence Lifetime in Gliomas with Different Tumor Grades, Intratumoral Regions, and Visible Fluorescence Status.Diagnostics (Basel, Switzerland) · 2024Article
- Development, Implementation and Application of Confocal Laser Endomicroscopy in Brain, Head and Neck Surgery-A Review.Diagnostics (Basel, Switzerland) · 2022Review
- Turning on the light for brain tumor surgery: A 5-aminolevulinic acid story.Neuro-oncology · 2022Review
- Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas.Frontiers in medicine · 2022Article
- Fluorescence Lifetime Imaging and Spectroscopic Co-Validation for Protoporphyrin IX-Guided Tumor Visualization in Neurosurgery.Frontiers in oncology · 2021Article
- Confocal Laser Endomicroscopy Assessment of Pituitary Tumor Microstructure: A Feasibility Study.Journal of clinical medicine · 2020Article
- Visualization technologies for 5-ALA-based fluorescence-guided surgeries.Journal of neuro-oncology · 2019Review
- Toward Quantitative Neurosurgical Guidance With High-Resolution Microscopy of 5-Aminolevulinic Acid-Induced Protoporphyrin IX.Frontiers in oncology · 2019Article
- Optical Characterization of Neurosurgical Operating Microscopes: Quantitative Fluorescence and Assessment of PpIX Photobleaching.Scientific reports · 2018Article
- Scanning Fiber Endoscope Improves Detection of 5-Aminolevulinic Acid-Induced Protoporphyrin IX Fluorescence at the Boundary of Infiltrative Glioma.World neurosurgery · 2018Article
- Optical-sectioning microscopy of protoporphyrin IX fluorescence in human gliomas: standardization and quantitative comparison with histology.Journal of biomedical optics · 2017Article
- Handheld confocal laser endomicroscopic imaging utilizing tumor-specific fluorescent labeling to identify experimental glioma cellsSurgical neurology international · 2016Article
- Video-rate in vivo fluorescence imaging with a line-scanned dual-axis confocal microscope.Journal of biomedical optics · 2015Article
- Dual-axis confocal microscopy for point-of-care pathology.IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics SocietyArticle
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
BACKGROUND AND
objectivesFluorescence image-guided surgery (FIGS), with contrast provided by 5-ALA-induced PpIX, has been shown to enable a higher extent of resection of high-grade gliomas. However, conventional FIGS with low-power microscopy lacks the sensitivity to aid in low-grade glioma (LGG) resection because PpIX signal is weak and sparse in such tissues. Intraoperative high-resolution microscopy of PpIX fluorescence has been proposed as a method to guide LGG resection, where sub-cellular resolution allows for the visualization of sparse and punctate mitochondrial PpIX production in tumor cells. Here, we assess the performance of three potentially portable high-resolution microscopy techniques that may be used for the intraoperative imaging of human LGG tissue samples with PpIX contrast: high-resolution fiber-optic microscopy (HRFM), high-resolution wide-field microscopy (WFM), and dual-axis confocal (DAC) microscopy. MATERIALS AND
methodsThick unsectioned human LGG tissue samples (n = 7) with 5-ALA-induced PpIX contrast were imaged using three imaging techniques (HRFM, WFM, DAC). The average signal-to-background ratio (SBR) was then calculated for each imaging modality (5 images per tissue, per modality).
resultsHRFM provides the ease of use and portability of a flexible fiber bundle, and is simple and inexpensive to build. However, in most cases (6/7), HRFM is not capable of detecting PpIX signal from LGGs due to high autofluorescence, generated by the fiber bundle under laser illumination at 405 nm, which overwhelms the PpIX signal and impedes its visualization. WFM is a camera-based method possessing high lateral resolution but poor axial resolution, resulting in sub-optimal image contrast.
conclusionsConsistent successful detection of PpIX signal throughout our human LGG tissue samples (n = 7), with an acceptable image contrast (SBR >2), was only achieved using DAC microscopy, which offers superior image resolution and contrast that is comparable to histology, but requires a laser-scanning mechanism to achieve optical sectioning.
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