ArticleScientific reports2015
Phenotypic and functional characterization of long-term cryopreserved human adipose-derived stem cells.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 2 of them syntheses that pooled it.
What it found
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The trial behind it
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Who cites it
45 citing papers in PubMed, 2 syntheses or guidelines pooled it, 111 citations in OpenAlex.
- Unveiling the phenotypic impact of cryopreservation on adipose derived stem cells: A systematic review.The Indian journal of medical research · 2025Pooled it
- Pooled it
- Advancing urine-derived stem cells: Cryopreservation validation and sex-specific metabolism.European journal of clinical investigation · 2026Article
- A novel cryopreservation solution for adipose tissue based on metformin.Stem cell research & therapy · 2025Article
- Comparative analysis of cryopreserved adipose stem cells expanded in hollow fiber bioreactor versus conventional tissue culture flasks.Scientific reports · 2024Article
- Challenges in the processing and preservation of adipose-derived stem cell subpopulations for clinical use.Regenerative medicine · 2024Article
- Orthobiologic Products: Preservation Options for Orthopedic Research and Clinical Applications.Journal of clinical medicine · 2024Review
- Effects, methods and limits of the cryopreservation on mesenchymal stem cells.Stem cell research & therapy · 2024Review
- Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities.RSC medicinal chemistry · 2024Review
- Exploring the Potential Effects of Cryopreservation on the Biological Characteristics and Cardiomyogenic Differentiation of Rat Adipose-Derived Mesenchymal Stem Cells.International journal of molecular sciences · 2024Article
- An Update on Adipose-Derived Stem Cells for Regenerative Medicine: Where Challenge Meets Opportunity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Comparison of Adipocyte Viability After Short-Term Cryopreservation of Adipose Aspirates Through 3 Different Techniques.Aesthetic surgery journal. Open forum · 2023Article
- Cryopreservation: A Review Article.Cureus · 2022Review
- Biomolecular Pathways of Cryoinjuries in Low-Temperature Storage for Mammalian Specimens.Bioengineering (Basel, Switzerland) · 2022Review
- Safety of Lavender Oil-Loaded Niosomes for In Vitro Culture and Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2022Article
- Article
- Stimulation of Metabolic Activity and Cell Differentiation in Osteoblastic and Human Mesenchymal Stem Cells by a Nanohydroxyapatite Paste Bone Graft Substitute.Materials (Basel, Switzerland) · 2022Article
- Enhanced Cellular Cryopreservation by Biopolymer-Associated Suppression of RhoA/ROCK Signaling Pathway.Materials (Basel, Switzerland) · 2021Article
- Overview of current adipose-derived stem cell (ADSCs) processing involved in therapeutic advancements: flow chart and regulation updates before and after COVID-19.Stem cell research & therapy · 2021Review
- Osteogenic differentiation potential and quantification of fresh and cryopreserved dental follicular stem cells-anJournal of stem cells & regenerative medicine · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryopreservation represents an effective technique to maintain the functional properties of human adipose-derived stem cells (ASCs) and allows pooling of cells via long-term storage for clinical applications, e.g., cell-based therapies. It is crucial to reduce freezing injury during the cryopreservation process by loading the ASCs with the optimum concentration of suitable cryoprotective agents (CPAs). In this study, human ASCs were preserved for 3 months in different combinations of CPAs, including 1) 0.25 M trehalose; 2) 5% dimethylsulfoxide (DMSO); 3) 10% DMSO; 4) 5% DMSO + 20% fetal bovine serum (FBS); 5) 10% DMSO + 20% FBS; 6) 10% DMSO + 90% FBS. Interestingly, even with a reduction of DMSO to 5% and without FBS, cryopreserved ASCs maintained high cell viability comparable with standard cryomedium (10% DMSO + 90% FBS), with normal cell phenotype and proliferation rate. Cryopreserved ASCs also maintained their differentiation capability (e.g., to adipocytes, osteocytes and chondrocytes) and showed an enhanced expression level of stemness markers (e.g., NANOG, OCT-4, SOX-2 and REX-1). Our findings suggest that 5% DMSO without FBS may be an ideal CPA for an efficient long-term cryopreservation of human ASCs. These results aid in establishing standardized xeno-free long-term cryopreservation of human ASCs for clinical applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.