ArticleClinica chimica acta; international journal of clinical chemistry2015
A high-performance liquid chromatography-tandem mass spectrometry-based targeted metabolomics kidney dysfunction marker panel in human urine.
Article in Clinica chimica acta; international journal of clinical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Targeted Livestock Metabolomics: A Review of Liquid chromatography-Mass Spectrometry-Based Approaches.Journal of agricultural and food chemistry · 2026Review
- Mass Spectrometry as a Tool for Metabolomics in Cancer.Advances in experimental medicine and biology · 2026Review
- Altered Arginine Metabolism in Children Undergoing Fontan Palliation: A Prospective Cohort Study.Pulmonary circulation · 2025Article
- Monitoring of Allograft Adaptation After Kidney Transplantation in Pediatric Patients by Targeted Plasma Metabolomics.International journal of molecular sciences · 2025Article
- Arginine-NO metabolites are associated with morbidity in single ventricle infants undergoing stage 2 palliation.Pediatric research · 2024Article
- The Role of Metabolomics and Microbiology in Urinary Tract Infection.International journal of molecular sciences · 2024Review
- Interstage Single Ventricle Heart Disease Infants Show Dysregulation in Multiple Metabolic Pathways: Targeted Metabolomics Analysis.JACC. Advances · 2023Article
- Article
- Sorbitol accumulation decreases oocyte quality in aged mice by altering the intracellular redox balance.Aging · 2021Article
- Advances in Detection of Kidney Transplant Injury.Molecular diagnosis & therapy · 2019Review
- Biomarkers in Transplantation--Proteomics and Metabolomics.Therapeutic drug monitoring · 2016Review
- Establishment of a Method for Measuring Antioxidant Capacity in Urine, Based on Oxidation Reduction Potential and Redox Couple IBioinorganic chemistry and applications · 2016Article
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5 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPrevious studies have examined and documented fluctuations in urine metabolites in response to disease processes and drug toxicity affecting glomerular filtration, tubule cell metabolism, reabsorption, oxidative stress, purine degradation, active secretion and kidney amino acylase activity representative of diminished renal function. However, a high-throughput assay that incorporates metabolites that are surrogate markers for such changes into a kidney dysfunction panel has yet to be described.
methodsA high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay for the quantification of ten metabolites associated with the Krebs cycle, purine degradation, and oxidative stress in human urine was developed and validated. Normal values were assessed in healthy adult (n=120) and pediatric (n=36) individuals. In addition, 9 pediatric renal transplant recipients patients were evaluated before and after initial dosing of the immunosuppressant tacrolimus in a proof-of-concept study.
resultsThe assay met all predefined acceptance criteria. The lower limit of quantification ranged from 0.1 to 1000 μmol/l. Inter-day trueness and imprecisions ranged from 91.4-112.9% and 1.5-12.4%, respectively. The total assay run time was 5.5 minutes. Concentrations of glucose, sorbitol, and trimethylamine oxide (TMAO) were elevated in pediatric renal transplant patients (n=9) prior to transplantation as well as before and immediately after initial dosing of tacrolimus. One month post-transplant urine metabolite patterns matched those of healthy children (n=36).
conclusionsThe LC-MS/MS assay will provide the basis for further large-scale clinical studies to explore these analytes as molecular markers for the patients with renal insufficiency.
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