ArticleCell journal2015
Brief azacytidine step allows the conversion of suspension human fibroblasts into neural progenitor-like cells.
Article in Cell journal, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Skin aging: mechanisms, evaluation, and rejuvenation.The EMBO journal · 2026Review
- Decellularized testicular bio-scaffolds driveFrontiers in bioengineering and biotechnology · 2026Article
- Use of Epigenetic Cues and Mechanical Stimuli to Generate Blastocyst-Like Structures from Mammalian Skin Dermal Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Bioengineering-tissue strategies to model mammalian implantationFrontiers in bioengineering and biotechnology · 2024Article
- Azacitidine induced lung injury: report and contemporary discussion on diagnosis and management.Frontiers in oncology · 2024Review
- Article
- Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.Frontiers in veterinary science · 2021Article
- Adding a dimension to cell fate.Animal reproduction · 2020Article
- All roads lead to Rome: the many ways to pluripotency.Journal of assisted reproduction and genetics · 2020Review
- How to reprogram human fibroblasts to neurons.Cell & bioscience · 2020Review
- New tools for cell reprogramming and conversion: Possible applications to livestock.Animal reproduction · 2019Article
- Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts.Stem cell reviews and reports · 2019Article
- Epigenetic Erasing and Pancreatic Differentiation of Dermal Fibroblasts into Insulin-Producing Cells are Boosted by the Use of Low-Stiffness Substrate.Stem cell reviews and reports · 2018Article
- How to Diagnose Early 5-Azacytidine-Induced Pneumonitis: A Case Report.Drug safety - case reports · 2017Article
- Induced dopaminergic neurons: A new promise for Parkinson's disease.Redox biology · 2017Article
- Neural Progenitor-Like Cells Induced from Human Gingiva-Derived Mesenchymal Stem Cells Regulate Myelination of Schwann Cells in Rat Sciatic Nerve Regeneration.Stem cells translational medicine · 2017Article
- 5-azacytidine affects TET2 and histone transcription and reshapes morphology of human skin fibroblasts.Scientific reports · 2016Article
- Chemical transdifferentiation: closer to regenerative medicine.Frontiers of medicine · 2016Review
- Erase and Rewind: Epigenetic Conversion of Cell Fate.Stem cell reviews and reports · 2016Review
- Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years transdifferentiation technology has enabled direct conversion of human fibroblasts to become a valuable, abundant and accessible cell source for patient-specific induced cell generation in biomedical research. The majority of transdifferentiation approaches rely upon viral gene delivery which due to random integration with the host genome can cause genome instability and tumorigenesis upon transplantation. Here, we provide a simple way to induce neural progenitor-like cells from human fibroblasts without genetic manipulation by changing physicochemical culture properties from monolayer culture into a suspension in the presence of a chemical DNA methyltransferase inhibitor agent, Azacytidine. We have demonstrated the expression of neural progenitor-like markers, morphology and the ability to spontaneously differentiate into neural-like cells. This approach is simple, inexpensive, lacks genetic manipulation and could be a foundation for future chemical neural transdifferentiation and a safe induction of neural progenitor cells from human fibroblasts for clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.