ArticleNucleic acids research2015
Functional interplay between the RK motif and linker segment dictates Oct4-DNA recognition.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
- caRBP-Pred: Leveraging Protein Language Models for the Prediction of Chromatin-Associated RNA-Binding Proteins.Computational and structural biotechnology journal · 2026Article
- Fuzzy protein-DNA interactions and beyond: A common theme in transcription?Current opinion in structural biology · 2024Review
- Structural mechanism of LIN28B nucleosome targeting by OCT4.Molecular cell · 2023Article
- Anti-ovarian cancer actions and pharmacological targets of plumbagin.Naunyn-Schmiedeberg's archives of pharmacology · 2023Article
- The homeodomain of Oct4 is a dimeric binder of methylated CpG elements.Nucleic acids research · 2023Article
- Structural mechanism ofbioRxiv : the preprint server for biology · 2023Article
- Key features of the POU transcription factor Oct4 from an evolutionary perspective.Cellular and molecular life sciences : CMLS · 2021Review
- Dissecting OCT4 defines the role of nucleosome binding in pluripotency.Nature cell biology · 2021Article
- Biological importance of OCT transcription factors in reprogramming and development.Experimental & molecular medicine · 2021Review
- LSD1-mediated demethylation of OCT4 safeguards pluripotent stem cells by maintaining the transcription of PORE-motif-containing genes.Scientific reports · 2021Article
- Diversity among POU transcription factors in chromatin recognition and cell fate reprogramming.Cellular and molecular life sciences : CMLS · 2018Review
- Molecular basis of CENP-C association with the CENP-A nucleosome at yeast centromeres.Genes & development · 2017Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The POU family transcription factor Oct4 plays pivotal roles in regulating pluripotency and somatic cell reprogramming. Previous studies have indicated an important role for major groove contacts in Oct4-DNA recognition; however, the contributions of the RK motif in the POUh domain and the linker segment joining the two DNA-binding domains remain poorly understood. Here, by combining molecular modelling and functional assays, we find that the RK motif is essential for Oct4-DNA association by recognizing the narrowed DNA minor groove. Intriguingly, computational simulations reveal that the function of the RK motif may be finely tuned by H-bond interactions with the partially disordered linker segment and that breaking these interactions significantly enhances the DNA binding and reprogramming activities of Oct4. These findings uncover a self-regulatory mechanism for specific Oct4-DNA recognition and provide insights into the functional crosstalk at the molecular level that may illuminate mechanistic studies of the Oct protein family and possibly transcription factors in the POU family. Our gain-of-function Oct4 mutants might also be useful tools for use in reprogramming and regenerative medicine.
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