ArticleNucleic acids research2015
A serine-arginine-rich (SR) splicing factor modulates alternative splicing of over a thousand genes in Toxoplasma gondii.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Translational control of Toxoplasma gondii differentiation.Bioscience reports · 2026Review
- A tale of two parasites: a glimpse into the RNA methylome of patient-derived Plasmodium falciparum and Plasmodium vivax isolates.Malaria journal · 2025Article
- A type II protein arginine methyltransferase regulates merozoite invasion in Plasmodium falciparum.Communications biology · 2023Article
- The splicing factor SR2 is an important virulence factor ofFrontiers in microbiology · 2023Article
- Regulator of calcineurin 1 is a novel RNA-binding protein to regulate neuronal apoptosis.Molecular psychiatry · 2021Article
- Direct Nanopore Sequencing of mRNA Reveals Landscape of Transcript Isoforms in Apicomplexan Parasites.mSystems · 2021Article
- Malaria parasites regulate intra-erythrocytic development duration via serpentine receptor 10 to coordinate with host rhythms.Nature communications · 2020Article
- Contribution of introns to the species diversity associated with the apicomplexan parasite, Neospora caninum.Parasitology research · 2020Article
- Alternative splicing is required for stage differentiation in malaria parasites.Genome biology · 2019Article
- Review
- The alternative splicing ofPlant signaling & behavior · 2019Article
- iTRAQ-Based Global Phosphoproteomics Reveals Novel Molecular Differences BetweenFrontiers in cellular and infection microbiology · 2019Article
- Comparative transcriptomics of female and male gametocytes in Plasmodium berghei and the evolution of sex in alveolates.BMC genomics · 2017Article
- Identified of a novel cis-element regulating the alternative splicing of LcDREB2.Scientific reports · 2017Article
- Comparative Monomethylarginine Proteomics Suggests that Protein Arginine Methyltransferase 1 (PRMT1) is a Significant Contributor to Arginine Monomethylation inMolecular & cellular proteomics : MCP · 2017Article
- Genome-Wide Transcriptome Analysis Reveals Extensive Alternative Splicing Events in the Protoscoleces ofFrontiers in microbiology · 2017Article
- Detection and visualization of differential splicing in RNA-Seq data with JunctionSeq.Nucleic acids research · 2016Article
- A Lipolytic Lecithin:Cholesterol Acyltransferase Secreted by Toxoplasma Facilitates Parasite Replication and Egress.The Journal of biological chemistry · 2016Article
- Alternative splicing mechanisms orchestrating post-transcriptional gene expression: intron retention and the intron-rich genome of apicomplexan parasites.Current genetics · 2016Article
- Article
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single genes are often subject to alternative splicing, which generates alternative mature mRNAs. This phenomenon is widespread in animals, and observed in over 90% of human genes. Recent data suggest it may also be common in Apicomplexa. These parasites have small genomes, and economy of DNA is evolutionarily favoured in this phylum. We investigated the mechanism of alternative splicing in Toxoplasma gondii, and have identified and localized TgSR3, a homologue of ASF/SF2 (alternative-splicing factor/splicing factor 2, a serine-arginine-rich, or SR protein) to a subnuclear compartment. In addition, we conditionally overexpressed this protein, which was deleterious to growth. qRT-PCR was used to confirm perturbation of splicing in a known alternatively-spliced gene. We performed high-throughput RNA-seq to determine the extent of splicing modulated by this protein. Current RNA-seq algorithms are poorly suited to compact parasite genomes, and hence we complemented existing tools by writing a new program, GeneGuillotine, that addresses this deficiency by segregating overlapping reads into distinct genes. In order to identify the extent of alternative splicing, we released another program, JunctionJuror, that detects changes in intron junctions. Using this program, we identified about 2000 genes that were constitutively alternatively spliced in T. gondii. Overexpressing the splice regulator TgSR3 perturbed alternative splicing in over 1000 genes.
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