ArticleProceedings of the National Academy of Sciences of the United States of America2015
Membrane fusion during phage lysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 93 citations in OpenAlex.
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- A conserved hub protein required for peptidoglycan remodeling and cell division inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Phage N4 uses a SAR endolysin-holin system for host cell lysis.bioRxiv : the preprint server for biology · 2025Article
- Therapeutic potential of Shigella phage SSG23 against Shigella sonnei biofilms and in BALB/c mice.Npj viruses · 2025Article
- TisB enables antibiotic tolerance in Salmonella by preventing prophage induction through ATP depletion.PLoS pathogens · 2025Article
- Characterization and Genomic Analysis of a Lytic Vibriophage vB_VneS_S3 of Infecting Vibrio neocaledonicus.Current microbiology · 2025Article
- Genetic insights into novel lysis suppression by phage CSP1 in Escherichia coli.Journal of microbiology (Seoul, Korea) · 2025Article
- Assessment of the phi6 lysis system using genetic complementation and heterologous expression inFrontiers in microbiology · 2025Article
- Preclinical characterization and in silico safety assessment of three virulent bacteriophages targeting carbapenem-resistant uropathogenic Escherichia coli.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Molecular mechanisms of precise timing in cell lysis.Biophysical journal · 2024Article
- Identifying components of theJournal of bacteriology · 2024Article
- Benefits and Challenges of Applying Bacteriophage Biocontrol in the Consumer Water Cycle.Microorganisms · 2024Review
- Co-ordinated assembly of the multilayered cell envelope of Gram-negative bacteria.Current opinion in microbiology · 2024Review
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- Genome characterization of a multi-drug resistantFrontiers in microbiology · 2024Article
- Harnessing a T1 Phage-Derived Spanin for Developing Phage-Based Antimicrobial Development.Biodesign research · 2024Article
- Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key Players Orchestrating Bacteriophage-Induced Cell Lysis and their Therapeutic Applications.Protein and peptide letters · 2024Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
In general, phages cause lysis of the bacterial host to effect release of the progeny virions. Until recently, it was thought that degradation of the peptidoglycan (PG) was necessary and sufficient for osmotic bursting of the cell. Recently, we have shown that in Gram-negative hosts, phage lysis also requires the disruption of the outer membrane (OM). This is accomplished by spanins, which are phage-encoded proteins that connect the cytoplasmic membrane (inner membrane, IM) and the OM. The mechanism by which the spanins destroy the OM is unknown. Here we show that the spanins of the paradigm coliphage lambda mediate efficient membrane fusion. This supports the notion that the last step of lysis is the fusion of the IM and OM. Moreover, data are provided indicating that spanin-mediated fusion is regulated by the meshwork of the PG, thus coupling fusion to murein degradation by the phage endolysin. Because endolysin function requires the formation of μm-scale holes by the phage holin, the lysis pathway is seen to require dramatic dynamics on the part of the OM and IM, as well as destruction of the PG.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.