ArticleInfection and immunity2015
The Type VI Secretion System Modulates Flagellar Gene Expression and Secretion in Citrobacter freundii and Contributes to Adhesion and Cytotoxicity to Host Cells.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- Prolonged Stagnation Reduces Treated Wastewater Biostability by Altering Microbial Community: Insights From Metaproteomics.Environmental microbiology · 2026Article
- Neisseria subflava Type 6 Secretion System competition with bacterial and fungal species.Applied microbiology and biotechnology · 2026Article
- Pan-genome insights into type VI secretion systems and their functional repertoires inmSystems · 2026Article
- TheInternational journal of molecular sciences · 2026Review
- T6SS1 suppresses pro-inflammatory cytokine transcription to drive immune evasion and systemic infection inInfection and immunity · 2026Article
- From past to present: the crosstalk between type VI secretion system of Salmonella and host.Archives of microbiology · 2025Review
- Occurrence of virulence factors and antimicrobial susceptibility ofJournal of veterinary research · 2025Article
- TssL2 of T6SS2 is required for mobility, biofilm formation, wrinkly phenotype formation, and virulence of Vibrio parahaemolyticus SH112.Applied microbiology and biotechnology · 2024Article
- The Role of ClpV in the Physiology and Pathogenicity ofMicroorganisms · 2024Article
- Characterization of cytotoxic Citrobacter braakii isolated from human stomach.FEBS open bio · 2024Article
- Flagella are required to coordinately activate competition and host colonization factors in response to a mechanical signal.bioRxiv : the preprint server for biology · 2024Article
- TagP, a PAAR-domain containing protein, plays roles in the fitness and virulence ofFrontiers in cellular and infection microbiology · 2024Article
- Roles of Hcp2, a Hallmark of T6SS2 in Motility, Adhesive Capacity, and Pathogenicity ofMicroorganisms · 2023Article
- Comparative Genomic Analyses of Virulence and Antimicrobial Resistance inMicroorganisms · 2023Article
- Diversity of the type VI secretion systems in theMicrobial genomics · 2023Article
- Endometrial microbiota in women with and without adenomyosis: A pilot study.Frontiers in microbiology · 2023Article
- A brief insight intoFrontiers in antibiotics · 2023Review
- H3-T6SS of Pseudomonas aeruginosa PA14 contributes to environmental adaptation via secretion of a biofilm-promoting effector.Stress biology · 2022Article
- Effect of the Type VI Secretion System Secreted Protein Hcp on the Virulence ofMicroorganisms · 2022Article
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The type VI secretion system (T6SS) as a virulence factor-releasing system contributes to virulence development of various pathogens and is often activated upon contact with target cells. Citrobacter freundii strain CF74 has a complete T6SS genomic island (GI) that contains clpV, hcp-2, and vgr T6SS genes. We constructed clpV, hcp-2, vgr, and T6SS GI deletion mutants in CF74 and analyzed their effects on the transcriptome overall and, specifically, on the flagellar system at the levels of transcription and translation. Deletion of the T6SS GI affected the transcription of 84 genes, with 15 and 69 genes exhibiting higher and lower levels of transcription, respectively. Members of the cell motility class of downregulated genes of the CF74ΔT6SS mutant were mainly flagellar genes, including effector proteins, chaperones, and regulators. Moreover, the production and secretion of FliC were also decreased in clpV, hcp-2, vgr, or T6SS GI deletion mutants in CF74 and were restored upon complementation. In swimming motility assays, the mutant strains were found to be less motile than the wild type, and motility was restored by complementation. The mutant strains were defective in adhesion to HEp-2 cells and were restored partially upon complementation. Further, the CF74ΔT6SS, CF74ΔclpV, and CF74Δhcp-2 mutants induced lower cytotoxicity to HEp-2 cells than the wild type. These results suggested that the T6SS GI in CF74 regulates the flagellar system, enhances motility, is involved in adherence to host cells, and induces cytotoxicity to host cells. Thus, the T6SS plays a wide-ranging role in C. freundii.
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