ArticleInfection and immunity2015
Phenotypic Heterogeneity in Expression of the K1 Polysaccharide Capsule of Uropathogenic Escherichia coli and Downregulation of the Capsule Genes during Growth in Urine.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Article
- Pathogen species and capsule expression shape opportunistic transfer and the recipient response during plasmid RP4 conjugation.Communications biology · 2026Article
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- Preclinical validation of anMicrobiology spectrum · 2024Article
- A convergent evolutionary pathway attenuating cellulose production drives enhanced virulence of some bacteria.Nature communications · 2024Article
- Characterization and comparative analysis of the Escherichia marmotae M-12 isolate from bank vole (Myodes glareolus).Scientific reports · 2023Article
- Phage Targeting Neonatal MeningitisInternational journal of molecular sciences · 2023Article
- Evolutionary and functional history of the Escherichia coli K1 capsule.Nature communications · 2023Article
- Transposable Element Insertions into the Escherichia coli Polysialic Acid Gene Cluster Result in Resistance to the K1F Bacteriophage.Microbiology spectrum · 2022Article
- Human Monoclonal Antibodies to Escherichia coli Outer Membrane Protein A Porin Domain Cause Aggregation but Do Not AlterInfection and immunity · 2022Article
- YbdO Promotes the Pathogenicity ofInternational journal of molecular sciences · 2022Article
- Regulation ofFrontiers in microbiology · 2022Review
- The Short-Chain Fatty Acids Propionate and Butyrate Augment Adherent-Invasive Escherichia coli Virulence but Repress Inflammation in a Human Intestinal Enteroid Model of Infection.Microbiology spectrum · 2021Article
- Article
- Super-Resolution Fluorescence Microscopy Study of the Production of K1 Capsules by Escherichia coli: Evidence for the Differential Distribution of the Capsule at the Poles and the Equator of the Cell.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Engineered K1F bacteriophages kill intracellular Escherichia coli K1 in human epithelial cells.Scientific reports · 2018Article
- Genetic exchanges are more frequent in bacteria encoding capsules.PLoS genetics · 2018Article
- Multidrug resistant pathogens respond differently to the presence of co-pathogen, commensal, probiotic and host cells.Scientific reports · 2018Article
- AutA and AutR, Two Novel Global Transcriptional Regulators, Facilitate Avian Pathogenic Escherichia coli Infection.Scientific reports · 2016Article
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Authors and funding
4 authors.
Funding
Abstract
Uropathogenic Escherichia coli (UPEC) is the major causative agent of uncomplicated urinary tract infections (UTI). The K1 capsule on the surface of UPEC strains is a key virulence factor, and its expression may be important in the onset and progression of UTI. In order to understand capsule expression in more detail, we analyzed its expression in the UPEC strain UTI89 during growth in rich medium (LB medium) and urine and during infection of a bladder epithelial cell line. Comparison of capsule gene transcription using a chromosomal gfp reporter fusion showed a significant reduction in transcription during growth in urine compared to that during growth in LB medium. When examined at the single-cell level, following growth in both media, capsule gene expression appears to be heterogeneous, with two distinct green fluorescent protein (GFP)-expressing populations. Using anti-K1 antibody, we showed that this heterogeneity in gene expression results in two populations of encapsulated and unencapsulated cells. We demonstrated that the capsule hinders attachment to and invasion of epithelial cells and that the unencapsulated cells within the population preferentially adhere to and invade bladder epithelial cells. We found that once internalized, UTI89 starts to produce capsule to aid in its intracellular survival and spread. We propose that this observed phenotypic diversity in capsule expression is a fitness strategy used by the bacterium to deal with the constantly changing environment of the urinary tract.
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