Evidence map›Paper›PMID 25870224›Full record

ArticleInfection and immunity2015

The role of CD1d-restricted NKT cells in the clearance of Pseudomonas aeruginosa from the lung is dependent on the host genetic background.

Patrick Benoit, Vaia Yioula Sigounas, Jenna L Thompson, Nico van Rooijen, Matthew E Poynter, Matthew J Wargo, Jonathan E Boyson

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Frontiers in immunology · 2024
    Review
  5. Article
  6. Article
  7. Contribution of Invariant Natural Killer T Cells to the Clearance ofInternational journal of molecular sciences · 2021
    Article
  8. Article
  9. Review
  10. Article
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Patrick BenoitDepartment of Surgery, University of Vermont College of Medicine, Burlington, Vermont, USA.
Vaia Yioula SigounasDepartment of Surgery, University of Vermont College of Medicine, Burlington, Vermont, USA.
Jenna L ThompsonDepartment of Microbiology and Molecular Genetics, University of Vermont College of Medicine, Burlington, Vermont, USA.
Nico van RooijenDepartment of Molecular Cell Biology, Free University Medical Center, Amsterdam, the Netherlands.
Matthew E PoynterDepartment of Medicine, University of Vermont College of Medicine, Burlington, Vermont, USA.
Matthew J WargoDepartment of Microbiology and Molecular Genetics, University of Vermont College of Medicine, Burlington, Vermont, USA.
Jonathan E BoysonDepartment of Surgery, University of Vermont College of Medicine, Burlington, Vermont, USA jboyson@uvm.edu.

Funding

Vermont Immunobioloigy/ Infectious Diseases CenterP20GM103496 · NIGMS · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BUDD, RALPH C · 2012 to 2015
$8.7M
CD1D-Restricted T cells and Pregnancy LossR01AI067897 · NIAID · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI BOYSON, JONATHAN E · 2007 to 2010
$1.5M
Serum Amyloid A3 in Allergic Asthma: An Endogenous Mediator of Disease SeverityR01HL107291 · NHLBI · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI POYNTER, MATTHEW E · 2012 to 2015
$1.5M
Pseudomonas detection and metabolism of sphingosineR01AI103003 · NIAID · UNIVERSITY OF VERMONT & ST AGRIC COLLEGE · PI WARGO, MATTHEW J · 2013 to 2017
$1.4M
NHLBI NIH HHS HL107291NHLBI NIH HHS R01 HL107291NIAID NIH HHS AI067897NIAID NIH HHS AI103003NIAID NIH HHS R01 AI067897NIAID NIH HHS R01 AI103003NIGMS NIH HHS GM103496NIGMS NIH HHS P20 GM103496
6 · The paper itself

Abstract

Pseudomonas aeruginosa is an important human opportunistic pathogen, accounting for a significant fraction of hospital-acquired lung infections. CD1d-restricted NKT cells comprise an unusual innate-like T cell subset that plays important roles in both bacterial and viral infections. Previous reports have differed in their conclusions regarding the role of NKT cells in clearance of P. aeruginosa from the lung. Since there is significant strain-dependent variation in NKT cell number and function among different inbred strains of mice, we investigated whether the role of NKT cells was dependent on the host genetic background. We found that NKT cells did indeed play a critical role in the clearance of P. aeruginosa from the lungs of BALB/c mice but that they played no discernible role in clearance from the lungs of C57BL/6 mice. We found that the strain-dependent role of NKT cells was associated with significant strain-dependent differences in cytokine production by lung NKT cells and that impaired clearance of P. aeruginosa in BALB/c CD1d(-/-) mice was associated with an increase in neutrophil influx to the lung and increased levels of proinflammatory cytokines and chemokines after infection. Finally, we found that the role of alveolar macrophages was also dependent on the genetic background. These data provide further support for a model in which the unusually high level of variability in NKT cell number and function among different genetic backgrounds may be an important contributor to infectious-disease susceptibility and pathology.

Indexed as

AnimalsAntigens, CD1dBronchoalveolar LavageCells, CulturedGene Expression RegulationHumansLungMacrophages, AlveolarMiceMice, Inbred BALB CMice, KnockoutNatural Killer T-CellsPneumonia, BacterialPseudomonas aeruginosaReceptors, Antigen, T-Cell, gamma-deltaAntigens, CD1dCD1d antigen, mouseReceptors, Antigen, T-Cell, gamma-delta

Identifiers

PMID25870224
PMCPMC4432766

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.