ArticleParasitology research2015
Acanthamoeba misidentification and multiple labels: redefining genotypes T16, T19, and T20 and proposal for Acanthamoeba micheli sp. nov. (genotype T19).
Article in Parasitology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 2 of them syntheses that pooled it.
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Who cites it
57 citing papers in PubMed, 2 syntheses or guidelines pooled it, 114 citations in OpenAlex.
- A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review.Memorias do Instituto Oswaldo Cruz · 2022Pooled it
- Genotype distribution of Acanthamoeba in keratitis: a systematic review.Parasitology research · 2021Pooled it
- Genome sequence of the protistMicrobiology resource announcements · 2026Article
- Anti-Scientifica · 2026Article
- Molecular Typing ofMicroorganisms · 2025Article
- Isolation and Molecular Identification of Pathogenic Free-Living Amoebae from Environmental Samples in Tenerife, Canary Islands, Spain.ACS ES&T water · 2025Article
- Effects of electroporation on Acanthamoeba Polyphaga.PloS one · 2025Article
- Comparative genomic analysis ofmSphere · 2024Article
- Learning from the rDNA Operon: A Reanalysis of theMicroorganisms · 2024Article
- Epidemiology of and Genetic Factors Associated withPathogens (Basel, Switzerland) · 2024Review
- Molecular detection and characterization of Acanthamoeba infection in dogs and its association with keratitis in Korea.Parasites, hosts and diseases · 2024Article
- On predatory fungi feeding on free-living amoebae harbouring yeast-like endoparasites.Parasitology research · 2023Article
- Molecular evidence for a new lineage within the Acanthamoeba T4 genotype.Parasitology research · 2023Article
- The Status of Molecular Analyses of Isolates ofMicroorganisms · 2023Review
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- An Insight into the Genome of Pathogenic and Non-PathogenicPathogens (Basel, Switzerland) · 2022Article
- Article
- Exploring LSU and ITS rDNA Sequences forMicroorganisms · 2022Article
- Real-Time PCR for the Diagnosis ofMicroorganisms · 2022Article
- Analysis of Acanthamoeba genotypes from public freshwater sources in Thailand reveals a new genotype, T23 Acanthamoeba bangkokensis sp. nov.Scientific reports · 2021Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acanthamoeba species are ubiquitous amoebae able to cause important infections in humans and other vertebrates. The full/near-full sequences (>2000 bp) of the small subunit ribosomal RNA gene (SSU rDNA or 18S rDNA) are used to cluster Acanthamoeba as genotypes, labeled T1 to T20. Genotype T15 remains an exception, being described only partially on a 1500-bp fragment. Strains are thus usually identified based on their 18S identity matches with reference strains, often using shorter (<500 bp) diagnostic fragments of the gene. Nevertheless, short fragments (<1000 bp) have been used to propose genotypes. This has been criticized, and doubts arise therefore on possible confusion leading to classify distinct partial sequences with a same label(s). We demonstrate herein that several partial sequences misassigned either to T16 or to T4, actually belong to at least two separate and distinct genotypes. We obtained the full 18S rDNA of a strain previously typed as T16 on the basis of a small fragment and demonstrated that it actually belongs to the recently described T19. We propose the name Acanthamoeba micheli sp. nov., for this strain. Furthermore, partial molecular phylogenies were performed to show that several other misassigned T16 partial sequences belong to a new genotype. This latter includes also misassigned T4 partial sequences, only recently available as full sequences and labeled as T20. We thus reassign these partial sequences to the genotype T20. Longer sequences, ideally at least 90 % of the total gene length, should be obtained from strains to ensure reliable diagnostic and phylogenetic results.
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