ArticleMarine biotechnology (New York, N.Y.)2015
Establishment of Genetic Transformation in the Sexually Reproducing Diatoms Pseudo-nitzschia multistriata and Pseudo-nitzschia arenysensis and Inheritance of the Transgene.
Article in Marine biotechnology (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 36 citations in OpenAlex.
- NPF2 is involved in intracellular pH regulation and ion balance in the diatom Phaeodactylum tricornutum.The New phytologist · 2026Article
- A genetic transformation system for the heterotrophic diatom Nitzschia putrida (Bacillariophyceae).Journal of phycology · 2025Article
- Genetic engineering in diatoms: advances and prospects.The Plant journal : for cell and molecular biology · 2025Review
- De novo transcriptome assembly of a lipoxygenase knock-down strain in the diatom Pseudo-nitzschia arenysensis.Scientific data · 2024Article
- Three Novel Bacteria Associated with Two Centric Diatom Species from the Mediterranean Sea,International journal of molecular sciences · 2021Article
- A Metabolomics Exploration of the Sexual Phase in the Marine DiatomMarine drugs · 2020Article
- Unveiling the presence of biosynthetic pathways for bioactive compounds in the Thalassiosira rotula transcriptome.Scientific reports · 2019Article
- Skeletonema marinoi as a new genetic model for marine chain-forming diatoms.Scientific reports · 2019Article
- MRP3 is a sex determining gene in the diatom Pseudo-nitzschia multistriata.Nature communications · 2018Article
- Genetic and metabolic engineering in diatoms.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Finding a partner in the ocean: molecular and evolutionary bases of the response to sexual cues in a planktonic diatom.The New phytologist · 2017Article
- The sexual phase of the diatom Pseudo-nitzschia multistriata: cytological and time-lapse cinematography characterization.Protoplasma · 2016Article
- Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum.Scientific reports · 2015Article
- Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum.Marine drugs · 2015Article
- Transcriptome sequencing of three Pseudo-nitzschia species reveals comparable gene sets and the presence of Nitric Oxide Synthase genes in diatoms.Scientific reports · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the genetic transformation of the planktonic diatoms Pseudo-nitzschia arenysensis and Pseudo-nitzschia multistriata, members of the widely distributed and ecologically important genus Pseudo-nitzschia. P. arenysensis and P. multistriata present the classical size reduction/restitution life cycle and can reproduce sexually. Genetic transformation was achieved with the biolistic method, using the H4 gene promoter from P. multistriata to drive expression of exogenous genes. The transformation was first optimized introducing the Sh ble gene to confer resistance to the antibiotic zeocin. Integration of the transgene was confirmed by PCR and Southern blot analyses. Subsequently, we simultaneously transformed in P. arenysensis two plasmids, one encoding the β-glucuronidase (GUS) gene together with the plasmid carrying the Sh ble resistance gene, demonstrating the possibility of co-transformation. By transforming a gene encoding a fusion between the histone H4 and the green fluorescent protein (GFP), we demonstrated that fluorescent tagging is possible and that studies for protein localization are feasible. Importantly, we crossed P. arenysensis- and P. multistriata-transformed strains with a wild-type strain of opposite mating type and demonstrated that the transgene can be inherited in the F1 generation. The possibility to transform two diatom species for which genetic crosses are possible opens the way to a number of new approaches, including classical loss of function screens and the possibility to obtain different combinations of double transformants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.