Evidence map›Paper›PMID 25869666›Full record

ArticleThe Journal of cell biology2015

Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways.

Kathleen Scheffler, Refael Minnes, Vincent Fraisier, Anne Paoletti, Phong T Tran

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

  1. Effects ofExperimental and therapeutic medicine · 2026
    Article
  2. Article
  3. Nuclear movement in fungi.Seminars in cell & developmental biology · 2018
    Review
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Kathleen SchefflerCentre de Recherche and BioImaging Cell and Tissue Core Facility of the Institut Curie (PICT-IBiSA), Institut Curie, F-75248 Paris, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France.
Refael MinnesCell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104.
Vincent FraisierCentre de Recherche and BioImaging Cell and Tissue Core Facility of the Institut Curie (PICT-IBiSA), Institut Curie, F-75248 Paris, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France.
Anne PaolettiCentre de Recherche and BioImaging Cell and Tissue Core Facility of the Institut Curie (PICT-IBiSA), Institut Curie, F-75248 Paris, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France.
Phong T TranCentre de Recherche and BioImaging Cell and Tissue Core Facility of the Institut Curie (PICT-IBiSA), Institut Curie, F-75248 Paris, France Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104 phong.tran@curie.fr.
Centre National de la Recherche Scientifique · FRInstitut Curie · FRUniversity of Pennsylvania · US

Funding

Mechanisms of spindle formationR01GM102215 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI TRAN, PHONG T · 2012 to 2015
$1.3M
NIGMS NIH HHS NIH R01GM102215NIGMS NIH HHS R01 GM102215
6 · The paper itself

Abstract

Microtubules (MTs) and associated motors play a central role in nuclear migration, which is crucial for diverse biological functions including cell division, polarity, and sexual reproduction. In this paper, we report a dual mechanism underlying nuclear congression during fission yeast karyogamy upon mating of haploid cells. Using microfluidic chambers for long-term imaging, we captured the precise timing of nuclear congression and identified two minus end-directed motors operating in parallel in this process. Kinesin-14 Klp2 associated with MTs may cross-link and slide antiparallel MTs emanating from the two nuclei, whereas dynein accumulating at spindle pole bodies (SPBs) may pull MTs nucleated from the opposite SPB. Klp2-dependent nuclear congression proceeds at constant speed, whereas dynein accumulation results in an increase of nuclear velocity over time. Surprisingly, the light intermediate chain Dli1, but not dynactin, is required for this previously unknown function of dynein. We conclude that efficient nuclear congression depends on the cooperation of two minus end-directed motors.

Indexed as

Cell NucleusDyneinsMicrotubule-Associated ProteinsMicrotubulesProtein TransportSchizosaccharomycesSchizosaccharomyces pombe ProteinsDhc1 protein, S pombeDyneinsKlp2 protein, S pombeMicrotubule-Associated ProteinsSchizosaccharomyces pombe Proteins

Identifiers

PMID25869666
PMCPMC4395489
OpenAlexW2084488780

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.