ArticleG3 (Bethesda, Md.)2015
Tools for Anopheles gambiae Transgenesis.
Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 134 citations in OpenAlex.
- Mosquito-based malaria control through genetic, microbial and immunological innovations.Nature reviews. Microbiology · 2026Review
- Article
- An OpIE2-DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector Anopheles gambiae.Genetics · 2026Article
- Compound effector genes suppress malaria parasite infections in gene-drive population modification strains of the African malaria mosquitoes, Anopheles gambiae and Anopheles coluzzii.G3 (Bethesda, Md.) · 2026Article
- Engineering resilient gene drives for sustainable malaria control by predicting, testing and overcoming target site resistance in Anopheles gambiae.PLoS biology · 2026Article
- Host serum-circulating flavivirus NS1 co-opts mosquito miRNA processing to suppress early immune defense.Nature communications · 2026Article
- Characterization of Anopheles gambiae immune cells through genetic and functional immunophenotyping.Nature communications · 2025Article
- Why just fly?Fly · 2025Review
- Article
- A male-drive female-sterile system for the self-limited control of the malaria mosquito Anopheles gambiae.Nature communications · 2025Article
- Engineering gene drive docking sites in a haplolethal locus in Anopheles gambiae.Scientific reports · 2025Article
- Dengue virus susceptibility in Aedes aegypti linked to natural cytochrome P450 promoter variants.Nature communications · 2025Article
- Targeting the mosquito prefoldin-chaperonin complex blocks Plasmodium transmission.Nature microbiology · 2025Article
- History of Mosquito Transgenesis: A Perspective in Review.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Exploring new dimensions of immune cell biology inbioRxiv : the preprint server for biology · 2024Article
- Anopheles gambiae lacking AgTRIO probe inefficiently on a mammalian host.Cell reports · 2024Article
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Targeting mosquito X-chromosomes reveals complex transmission dynamics of sex ratio distorting gene drives.Nature communications · 2024Article
- Y chromosome shredding in Anopheles gambiae: Insight into the cellular dynamics of a novel synthetic sex ratio distorter.PLoS genetics · 2024Article
- An expanded neurogenetic toolkit to decode olfaction in the African malaria mosquito Anopheles gambiae.Cell reports methods · 2024Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
Abstract
Transgenesis is an essential tool to investigate gene function and to introduce desired characters in laboratory organisms. Setting-up transgenesis in non-model organisms is challenging due to the diversity of biological life traits and due to knowledge gaps in genomic information. Some procedures will be broadly applicable to many organisms, and others have to be specifically developed for the target species. Transgenesis in disease vector mosquitoes has existed since the 2000s but has remained limited by the delicate biology of these insects. Here, we report a compilation of the transgenesis tools that we have designed for the malaria vector Anopheles gambiae, including new docking strains, convenient transgenesis plasmids, a puromycin resistance selection marker, mosquitoes expressing cre recombinase, and various reporter lines defining the activity of cloned promoters. This toolbox contributed to rendering transgenesis routine in this species and is now enabling the development of increasingly refined genetic manipulations such as targeted mutagenesis. Some of the reagents and procedures reported here are easily transferable to other nonmodel species, including other disease vector or agricultural pest insects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.