ArticleThe Journal of biological chemistry2015
Biochemical Validation of the Glyoxylate Cycle in the Cyanobacterium Chlorogloeopsis fritschii Strain PCC 9212.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 80 citations in OpenAlex.
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- Evaluation of Growth Performance, Biochemical Composition, and Polyhydroxyalkanoates Production of Four Cyanobacterial Species Grown in Cheese Whey.Microorganisms · 2025Article
- Sulfate assimilation regulates antioxidant defense response of the cyanobacteriumApplied and environmental microbiology · 2025Article
- Host-dependent specialized metabolism of nitrogen export in actinorhizal nodules induced by diazotrophic Actinomycetota Frankia cluster-2.Journal of experimental botany · 2025Article
- Insufficient Acetyl-CoA Pool Restricts the Phototrophic Production of Organic Acids in Model Cyanobacteria.International journal of molecular sciences · 2024Review
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- Identification of a Far-Red Light-Inducible Promoter that Exhibits Light Intensity Dependency and Reversibility in a Cyanobacterium.ACS synthetic biology · 2023Article
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- Comparative analysis of genome-based CAZyme cassette in Antarctic Microbacterium sp. PAMC28756 with 31 other Microbacterium species.Genes & genomics · 2022Article
- The novel genus, 'Candidatus Phosphoribacter', previously identified as Tetrasphaera, is the dominant polyphosphate accumulating lineage in EBPR wastewater treatment plants worldwide.The ISME journal · 2022Article
- Combined Impact of Magnetic Force and Spaceflight Conditions onInternational journal of molecular sciences · 2022Article
- Engineering the glyoxylate cycle for chemical bioproduction.Frontiers in bioengineering and biotechnology · 2022Review
- Production of succinate by engineered strains of Synechocystis PCC 6803 overexpressing phosphoenolpyruvate carboxylase and a glyoxylate shunt.Microbial cell factories · 2021Article
- Modifying the Cyanobacterial Metabolism as a Key to Efficient Biopolymer Production in Photosynthetic Microorganisms.International journal of molecular sciences · 2020Review
- Phosphoglycolate salvage in a chemolithoautotroph using the Calvin cycle.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Recent Advances in the Photoautotrophic Metabolism of Cyanobacteria: Biotechnological Implications.Life (Basel, Switzerland) · 2020Review
- Metabonomic strategy for the detection of metabolic effects of probiotics combined with prebiotic supplementation in weaned rats.RSC advances · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are important photoautotrophic bacteria with extensive but variable metabolic capacities. The existence of the glyoxylate cycle, a variant of the TCA cycle, is still poorly documented in cyanobacteria. Previous studies reported the activities of isocitrate lyase and malate synthase, the key enzymes of the glyoxylate cycle in some cyanobacteria, but other studies concluded that these enzymes are missing. In this study the genes encoding isocitrate lyase and malate synthase from Chlorogloeopsis fritschii PCC 9212 were identified, and the recombinant enzymes were biochemically characterized. Consistent with the presence of the enzymes of the glyoxylate cycle, C. fritschii could assimilate acetate under both light and dark growth conditions. Transcript abundances for isocitrate lyase and malate synthase increased, and C. fritschii grew faster, when the growth medium was supplemented with acetate. Adding acetate to the growth medium also increased the yield of poly-3-hydroxybutyrate. When the genes encoding isocitrate lyase and malate synthase were expressed in Synechococcus sp. PCC 7002, the acetate assimilation capacity of the resulting strain was greater than that of wild type. Database searches showed that the genes for the glyoxylate cycle exist in only a few other cyanobacteria, all of which are able to fix nitrogen. This study demonstrates that the glyoxylate cycle exists in a few cyanobacteria, and that this pathway plays an important role in the assimilation of acetate for growth in one of those organisms. The glyoxylate cycle might play a role in coordinating carbon and nitrogen metabolism under conditions of nitrogen fixation.
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