ArticleZeitschrift fur Rheumatologie2015
[CD15 focus score for diagnostics of periprosthetic joint infections : Neutrophilic granulocytes quantification mode and the development of morphometric software (CD15 quantifier)].
Article in Zeitschrift fur Rheumatologie, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 18 citations in OpenAlex.
- [Meeting report of the Bone, Joint and Soft Tissue Pathology Working Group : DGP Conference, 13 June 2019, Frankfurt].Der Pathologe · 2019Article
- General synovitis score and immunologic synovitis score reflect clinical disease activity in patients with advanced stage rheumatoid arthritis.Scientific reports · 2019Article
- [Histopathological classification principles of rheumatic joint diseases : Contribution of pathology to the diagnosis].Der Orthopade · 2018Review
- [Arthur Vick Prize 2017 of the German Society of Orthopaedic Rheumatology].Zeitschrift fur Rheumatologie · 2018Review
- CD3+ lymphocytosis in the peri-implant membrane of 222 loosened joint endoprostheses depends on the tribological pairing.Acta orthopaedica · 2017Article
- [Fifteen years of the histopathological synovitis score : Review and further developments of a diagnostic score].Zeitschrift fur Rheumatologie · 2017Review
- [Implant-associated infections - Diagnostics].Der Chirurg; Zeitschrift fur alle Gebiete der operativen Medizen · 2016Review
- [Histopathological diagnostic work-up of joint endoprosthesis-associated pathologies].Der Hautarzt; Zeitschrift fur Dermatologie, Venerologie, und verwandte Gebiete · 2016Review
- [Revision arthroplasty : Histopathological diagnostics in periprosthetic joint infections].Der Orthopade · 2015Article
- Histopathological Osteomyelitis Evaluation Score (HOES) - an innovative approach to histopathological diagnostics and scoring of osteomyelitis.GMS Interdisciplinary plastic and reconstructive surgery DGPW · 2014Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aim of this project was to devise a quantification method for neutrophils within a single focal point through the development of a CD15 focus score which enables bacterial infections in synovial-like interface membranes (SLIM) to be diagnosed.
methodsIn this study a histopathological classification of 91 SLIM removed during revision surgery from the hips (n = 59) and knees (n = 32) was performed. Neutrophils were identified immunohistochemically by means of a CD15-specific monoclonal antibody. The quantitative evaluation of CD15-positive neutrophils (CD15Ne) used the principle of maximum focal infiltration (focus) together with an assessment of a single focal point (0.3 mm(2)). This immunohistochemical approach made it possible to develop the CD15 quantifier software, which automatically quantifies CD15Ne.
resultsThe SLIM cases with positive microbiological findings (n = 47) had significantly (p < 0.001, Mann-Whitney U-test) more CD15Ne/focal point than cases with negative microbiological findings (n = 44). A count of 50 CD15Ne/focal point was identified as the optimum threshold when diagnosing periprosthetic joint infections (PJI) using the CD15 focus score. If the microbiological findings are used as a gold standard, the diagnostic sensitivity is 0.83, and the specificity is 0.864 with a positive predictive value (PPV) of 0.87, a negative predictive value (NPV) of 0.83, an accuracy of 0.846 and an area under the curve (AUC) of 0.878. The evaluation of findings for the preparations using the CD15 quantifier software (n = 31) deviated by an average of 12 cells from the histopathological evaluation findings (CD15 focus score). Above a cell count of 62, the CD15-quantifier needs on average 32 s less than the pathologist.
conclusionThe immunohistochemical CD15 focus score has a high diagnostic value and allowed the development of the CD15 quantifier software. This provides an automated procedure, which shortens the mentally tiring and time-consuming process of microscopic cell counting and thus makes a contribution towards the standardization of tools for diagnosing PJI.
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