ArticleJournal of visualized experiments : JoVE2015
Development of a quantitative recombinase polymerase amplification assay with an internal positive control.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Thermodynamically programmed one-pot CRISPR platform for point-of-care SNP genotyping.Nature communications · 2026Article
- Evaluation of recombinase polymerase amplification assays for targeted detection of bovine respiratory disease bacterial pathogens and antimicrobial-resistance genes in feedlot calves.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2026Article
- SARS-CoV-2 recombinase polymerase amplification assay with lateral flow readout and duplexed full process internal control.Sensors & diagnostics · 2024Article
- A novel tailed primer nucleic acid test for detection of HPV 16, 18 and 45 DNA at the point of care.Scientific reports · 2023Article
- Characterizing the Impact of Primer-Template Mismatches on Recombinase Polymerase Amplification.The Journal of molecular diagnostics : JMD · 2022Article
- Characterization of wax valving and μPIV analysis of microscale flow in paper-fluidic devices for improved modeling and design.Lab on a chip · 2022Article
- Sensitive, Single-Pot, Two-Stage Assay for Hepatitis Viruses.Analytical chemistry · 2022Article
- Sensitive and semiquantitative detection of soil-transmitted helminth infection in stool using a recombinase polymerase amplification-based assay.PLoS neglected tropical diseases · 2021Article
- Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.Biosensors · 2021Article
- Influence of design probe and sequence mismatches on the efficiency of fluorescent RPA.World journal of microbiology & biotechnology · 2019Article
- Development of real-time reverse transcription recombinase polymerase amplification (RPA) for rapid detection of peste des petits ruminants virus in clinical samples and its comparison with real-time PCR test.Scientific reports · 2018Article
- Article
- Development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens.Journal of applied microbiology · 2017Article
- Recombinase Polymerase Amplification for Diagnostic Applications.Clinical chemistry · 2016Review
- Factors influencing Recombinase polymerase amplification (RPA) assay outcomes at point of care.Molecular and cellular probes · 2016Article
- Cross-subtype detection of HIV-1 using reverse transcription and recombinase polymerase amplification.Journal of virological methods · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It was recently demonstrated that recombinase polymerase amplification (RPA), an isothermal amplification platform for pathogen detection, may be used to quantify DNA sample concentration using a standard curve. In this manuscript, a detailed protocol for developing and implementing a real-time quantitative recombinase polymerase amplification assay (qRPA assay) is provided. Using HIV-1 DNA quantification as an example, the assembly of real-time RPA reactions, the design of an internal positive control (IPC) sequence, and co-amplification of the IPC and target of interest are all described. Instructions and data processing scripts for the construction of a standard curve using data from multiple experiments are provided, which may be used to predict the concentration of unknown samples or assess the performance of the assay. Finally, an alternative method for collecting real-time fluorescence data with a microscope and a stage heater as a step towards developing a point-of-care qRPA assay is described. The protocol and scripts provided may be used for the development of a qRPA assay for any DNA target of interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.