ArticleJournal of visualized experiments : JoVE2015
Dried blood spots--preparing and processing for use in immunoassays and in molecular techniques.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT04871230 (Dried Blood Spot Test to Assess TB in Pregnancy in Remote and Resource Limited Areas), which is not on this map. Cited by 90 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Dried Blood Spot Test to Assess TB in Pregnancy in Remote and Resource Limited Areas
Who cites it
90 citing papers in PubMed, 3 syntheses or guidelines pooled it, 174 citations in OpenAlex.
- Reliability of dried blood spot (DBS) cards in antibody measurement: A systematic review.PloS one · 2021Pooled it
- Diagnostic accuracy of detection and quantification of HBV-DNA and HCV-RNA using dried blood spot (DBS) samples - a systematic review and meta-analysis.BMC infectious diseases · 2017Pooled it
- Diagnostic accuracy of serological diagnosis of hepatitis C and B using dried blood spot samples (DBS): two systematic reviews and meta-analyses.BMC infectious diseases · 2017Pooled it
- Article
- Article
- Does Capillary or Intravenous Collection of Dried Blood Spots Affect the Results of Amino Acid and Acylcarnitine Profile Studied with Tandem Mass Spectrometry?Metabolites · 2026Article
- Mapping Infant Immunity with Minimal Input: Integrative Single-cell and Multiomic Profiling.Journal of visualized experiments : JoVE · 2026Article
- Dietary intake, nutritional status and healthcare characteristics of mothers and newborn infants in a prospective cohort study (CHAMP) from a malnutrition-endemic region of Pakistan.Frontiers in nutrition · 2026Article
- The analytical potential of the dry blood spot technique for pesticide biomonitoring - a review.Environmental monitoring and assessment · 2025Review
- Serum-equivalency comparison, detection, and quantification of Group BHuman vaccines & immunotherapeutics · 2025Article
- Connecting the Dots: Bridging Microsamples and Conventional Blood Matrices in Metabolic Biomarker Analysis.Analytical science advances · 2025Review
- Validation on the First-Tier Fully Automated High-ThroughputInternational journal of neonatal screening · 2025Article
- Detection of Anti-IFNγ Autoantibodies From Dried Blood Spots.Open forum infectious diseases · 2025Article
- Article
- Performance of cellulose-based card for direct genetic testing of spinal muscular atrophy.BMC biotechnology · 2025Article
- Optimization of dried blood spot for hepatitis B virus surface antibody quantification.PloS one · 2025Article
- A single HIIT session does not alter blood sphingolipid levels in healthy young adults: The SphingoHIIT randomized controlled trial.Computational and structural biotechnology journal · 2025Article
- CFTR haplotype phasing using long-read genome sequencing from ultralow input DNA.Genetics in medicine open · 2025Article
- Progress and challenges in the elimination of hepatitis C among people who inject drugs in Germany: results of a pilot study for a national monitoring system, 10 years after the first data collection.Harm reduction journal · 2024Article
- Placental malaria and adverse pregnancy outcomes in Majang Zone of Gambella Region, Southwest Ethiopia: a histopathological and molecular study.Malaria journal · 2024Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The idea of collecting blood on a paper card and subsequently using the dried blood spots (DBS) for diagnostic purposes originated a century ago. Since then, DBS testing for decades has remained predominantly focused on the diagnosis of infectious diseases especially in resource-limited settings or the systematic screening of newborns for inherited metabolic disorders and only recently have a variety of new and innovative DBS applications begun to emerge. For many years, pre-analytical variables were only inappropriately considered in the field of DBS testing and even today, with the exception of newborn screening, the entire pre-analytical phase, which comprises the preparation and processing of DBS for their final analysis has not been standardized. Given this background, a comprehensive step-by-step protocol, which covers al the essential phases, is proposed, i.e., collection of blood; preparation of blood spots; drying of blood spots; storage and transportation of DBS; elution of DBS, and finally analyses of DBS eluates. The effectiveness of this protocol was first evaluated with 1,762 coupled serum/DBS pairs for detecting markers of hepatitis B virus, hepatitis C virus, and human immunodeficiency virus infections on an automated analytical platform. In a second step, the protocol was utilized during a pilot study, which was conducted on active drug users in the German cities of Berlin and Essen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.