ArticlePloS one2015
Identification of the avian RBP7 gene as a new adipose-specific gene and RBP7 promoter-driven GFP expression in adipose tissue of transgenic quail.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Heart-specific expression of the green fluorescent protein gene in avian embryos by administration of recombinant adenovirus type-5 vector into the embryonic blood vessel.Frontiers in physiology · 2024Article
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- Chordin-like 1, a Novel Adipokine, Markedly Promotes Adipogenesis and Lipid Accumulation.Cells · 2023Article
- Global Single-Cell Sequencing Landscape of Adipose Tissue of Different Anatomical Site Origin in Humans.Stem cells international · 2023Article
- Retinol Binding Protein 7 Promotes AdipogenesisFrontiers in cell and developmental biology · 2022Article
- Hypertrophy of Adipose Tissues in Quail Embryos byFrontiers in physiology · 2021Article
- Current Approaches and Applications in Avian Genome Editing.International journal of molecular sciences · 2020Review
- Transgenesis and web resources in quail.eLife · 2020Article
- Article
- Direct delivery of adenoviral CRISPR/Cas9 vector into the blastoderm for generation of targeted gene knockout in quail.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Integrative Analysis Revealing Human Adipose-Specific Genes and Consolidating Obesity Loci.Scientific reports · 2019Article
- Avian Bioreactor Systems: A Review.Molecular biotechnology · 2018Review
- Identification of differentially expressed genes and pathways for intramuscular fat metabolism between breast and thigh tissues of chickens.BMC genomics · 2018Article
- Article
- Identification of the MUC2 Promoter as a Strong Promoter for Intestinal Gene Expression through Generation of Transgenic Quail Expressing GFP in Gut Epithelial Cells.International journal of molecular sciences · 2017Article
- Advances in genetic engineering of the avian genome: "Realising the promise".Transgenic research · 2016Review
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The discovery of an increasing number of new adipose-specific genes has significantly contributed to our understanding of adipose tissue biology and the etiology of obesity and its related diseases. In the present study, comparison of gene expression profiles among various tissues was performed by analysis of chicken microarray data, leading to identification of RBP7 as a novel adipose-specific gene in chicken. Adipose-specific expression of RBP7 in the avian species was further confirmed at the protein and mRNA levels. Examination of the transcription factor binding sites within the chicken RBP7 promoter by Matinspector software revealed potential binding sites for adipogenic transcription factors. This led to the hypothesis that the RBP7 promoter can be utilized to overexpress a transgene in adipose tissue in order to further investigate the function of a transgene in adipose tissue. Several lines of transgenic quail containing a green fluorescent protein (GFP) gene under the control of the RBP7 promoter were generated using lentivirus-mediated gene transfer. The GFP expression in transgenic quail was specific to adipose tissue and increased after adipocyte differentiation. This expression pattern was consistent with endogenous RBP7 expression, suggesting the RBP7 promoter is sufficient to overexpress a gene of interest in adipose tissue at later developmental stages. These findings will lead to the establishment of a novel RBP7 promoter cassette which can be utilized for overexpressing genes of interest in adipose tissue in vivo to study the function of genes in adipose tissue development and lipid metabolism.
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