ArticleJournal of visualized experiments : JoVE2015
Techniques for the analysis of extracellular vesicles using flow cytometry.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Extracellular Vesicles in Allergy: From Cellular Communication to Clinical Implications.Clinical reviews in allergy & immunology · 2026Review
- Antigen Presentation by Extracellular Vesicles in the Acute Phase of Corneal Transplantation.Investigative ophthalmology & visual science · 2025Article
- Integrated SERS-Microfluidic Sensor Based on Nano-Micro Hierarchical Cactus-like Array Substrates for the Early Diagnosis of Prostate Cancer.Biosensors · 2024Article
- Extracellular vesicles in systemic juvenile idiopathic arthritis.Journal of leukocyte biology · 2023Article
- Pravastatin reduces plasma levels of extracellular vesicles in pregnancies at high risk of term preeclampsia.Frontiers in pharmacology · 2023Article
- CNS endothelial derived extracellular vesicles are biomarkers of active disease in multiple sclerosis.Fluids and barriers of the CNS · 2022Article
- Comparative analysis of extracellular vesicle isolation methods from human AML bone marrow cells and AML cell lines.Frontiers in oncology · 2022Article
- Article
- Zoom in on Antibody Aggregates: A Potential Pitfall in the Search of Rare EV Populations.Biomedicines · 2021Article
- Seminal Plasma-Derived Extracellular-Vesicle Fractions from HIV-Infected Men Exhibit Unique MicroRNA Signatures and Induce a Proinflammatory Response in Cells Isolated from the Female Reproductive Tract.Journal of virology · 2020Observational
- Antibody-Free Labeling of Malaria-Derived Extracellular Vesicles Using Flow Cytometry.Biomedicines · 2020Article
- Regulation of endothelial cell permeability by platelet-derived extracellular vesicles.The journal of trauma and acute care surgery · 2019Article
- CNS-derived extracellular vesicles from superoxide dismutase 1 (SOD1)The Journal of biological chemistry · 2019Article
- Blood manufacturing methods affect red blood cell product characteristics and immunomodulatory activity.Blood advances · 2018Article
- Routine Screening Method for Microparticles in Platelet Transfusions.Journal of visualized experiments : JoVE · 2018Article
- Molecular Detection and Analysis of Exosomes Using Surface-Enhanced Raman Scattering Gold Nanorods and a Miniaturized Device.Theranostics · 2018Article
- Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration.Journal of visualized experiments : JoVE · 2017Article
- Gold Nanoparticle Based Platforms for Circulating Cancer Marker Detection.Nanotheranostics · 2017Article
- Strategies for enumeration of circulating microvesicles on a conventional flow cytometer: Counting beads and scatter parameters.Journal of circulating biomarkersArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Extracellular Vesicles (EVs) are small, membrane-derived vesicles found in bodily fluids that are highly involved in cell-cell communication and help regulate a diverse range of biological processes. Analysis of EVs using flow cytometry (FCM) has been notoriously difficult due to their small size and lack of discrete populations positive for markers of interest. Methods for EV analysis, while considerably improved over the last decade, are still a work in progress. Unfortunately, there is no one-size-fits-all protocol, and several aspects must be considered when determining the most appropriate method to use. Presented here are several different techniques for processing EVs and two protocols for analyzing EVs using either individual detection or a bead-based approach. The methods described here will assist with eliminating the antibody aggregates commonly found in commercial preparations, increasing signal-to-noise ratio, and setting gates in a rational fashion that minimizes detection of background fluorescence. The first protocol uses an individual detection method that is especially well suited for analyzing a high volume of clinical samples, while the second protocol uses a bead-based approach to capture and detect smaller EVs and exosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.