ArticleJournal of visualized experiments : JoVE2015
Analysis of cardiomyocyte development using immunofluorescence in embryonic mouse heart.
Article in Journal of visualized experiments : JoVE, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Label-free method to monitor metabolism during long-term culture of human pluripotent stem cell derived cardiomyocytes.Biophotonics discovery · 2025Article
- Deletion of Sphingosine 1-Phosphate receptor 1 in cardiomyocytes during development leads to abnormal ventricular conduction and fibrosis.Physiological reports · 2021Article
- Ablation of SUN2-containing LINC complexes drives cardiac hypertrophy without interstitial fibrosis.Molecular biology of the cell · 2019Article
- Analysis of Coronary Vessels in Cleared Embryonic Hearts.Journal of visualized experiments : JoVE · 2016Article
- Sphingosine 1-phosphate receptor-1 in cardiomyocytes is required for normal cardiac development.Developmental biology · 2016Article
- Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
During heart development, the generation of myocardial-specific structural and functional units including sarcomeres, contractile myofibrils, intercalated discs, and costameres requires the coordinated assembly of multiple components in time and space. Disruption in assembly of these components leads to developmental heart defects. Immunofluorescent staining techniques are used commonly in cultured cardiomyocytes to probe myofibril maturation, but this ex vivo approach is limited by the extent to which myocytes will fully differentiate in culture, lack of normal in vivo mechanical inputs, and absence of endocardial cues. Application of immunofluorescence techniques to the study of developing mouse heart is desirable but more technically challenging, and methods often lack sufficient sensitivity and resolution to visualize sarcomeres in the early stages of heart development. Here, we describe a robust and reproducible method to co-immunostain multiple proteins or to co-visualize a fluorescent protein with immunofluorescent staining in the embryonic mouse heart and use this method to analyze developing myofibrils, intercalated discs, and costameres. This method can be further applied to assess cardiomyocyte structural changes caused by mutations that lead to developmental heart defects.
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Registered trials
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