Evidence map›Paper›PMID 25866874›Full record

ArticleMolecular endocrinology (Baltimore, Md.)2015

Evidence That Up-Regulation of MicroRNA-29 Contributes to Postnatal Body Growth Deceleration.

Fariha Kamran, Anenisia C Andrade, Aikaterini A Nella, Samuel J Clokie, Geoffrey Rezvani, Ola Nilsson, Jeffrey Baron, Julian C Lui

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular endocrinology (Baltimore, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Regulation of body growth by microRNAs.Molecular and cellular endocrinology · 2017
    Review
  10. MicroRNA-29: A Crucial Player in Fibrotic Disease.Molecular diagnosis & therapy · 2017
    Review
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Fariha KamranSection on Growth and Development (F.K., A.A.N., G.R., J.B., J.C.L.) and Section on Neuroendocrinology (S.J.C.), Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and Center for Molecular Medicine and Pediatric Endocrinology Unit (A.C.A., O.N.), Department of Women's and Children's Health, Karolinska Institutet and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
Anenisia C AndradeSection on Growth and Development (F.K., A.A.N., G.R., J.B., J.C.L.) and Section on Neuroendocrinology (S.J.C.), Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and Center for Molecular Medicine and Pediatric Endocrinology Unit (A.C.A., O.N.), Department of Women's and Children's Health, Karolinska Institutet and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
Aikaterini A NellaSection on Growth and Development (F.K., A.A.N., G.R., J.B., J.C.L.) and Section on Neuroendocrinology (S.J.C.), Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and Center for Molecular Medicine and Pediatric Endocrinology Unit (A.C.A., O.N.), Department of Women's and Children's Health, Karolinska Institutet and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
Samuel J ClokieSection on Growth and Development (F.K., A.A.N., G.R., J.B., J.C.L.) and Section on Neuroendocrinology (S.J.C.), Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and Center for Molecular Medicine and Pediatric Endocrinology Unit (A.C.A., O.N.), Department of Women's and Children's Health, Karolinska Institutet and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
Geoffrey RezvaniSection on Growth and Development (F.K., A.A.N., G.R., J.B., J.C.L.) and Section on Neuroendocrinology (S.J.C.), Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and Center for Molecular Medicine and Pediatric Endocrinology Unit (A.C.A., O.N.), Department of Women's and Children's Health, Karolinska Institutet and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
Ola NilssonSection on Growth and Development (F.K., A.A.N., G.R., J.B., J.C.L.) and Section on Neuroendocrinology (S.J.C.), Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and Center for Molecular Medicine and Pediatric Endocrinology Unit (A.C.A., O.N.), Department of Women's and Children's Health, Karolinska Institutet and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
Jeffrey BaronSection on Growth and Development (F.K., A.A.N., G.R., J.B., J.C.L.) and Section on Neuroendocrinology (S.J.C.), Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and Center for Molecular Medicine and Pediatric Endocrinology Unit (A.C.A., O.N.), Department of Women's and Children's Health, Karolinska Institutet and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
Julian C LuiSection on Growth and Development (F.K., A.A.N., G.R., J.B., J.C.L.) and Section on Neuroendocrinology (S.J.C.), Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892; and Center for Molecular Medicine and Pediatric Endocrinology Unit (A.C.A., O.N.), Department of Women's and Children's Health, Karolinska Institutet and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.
National Institutes of Health · USEunice Kennedy Shriver National Institute of Child Health and Human Development · USKarolinska Institutet · SEKarolinska University Hospital · SE

Funding

Regulation Of Childhood GrowthZIAHD000640 · NICHD · EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT · PI BARON, JEFFREY · 2009 to 2025
$24.4M
Intramural NIH HHS
6 · The paper itself

Abstract

Body growth is rapid in infancy but subsequently slows and eventually ceases due to a progressive decline in cell proliferation that occurs simultaneously in multiple organs. We previously showed that this decline in proliferation is driven in part by postnatal down-regulation of a large set of growth-promoting genes in multiple organs. We hypothesized that this growth-limiting genetic program is orchestrated by microRNAs (miRNAs). Bioinformatic analysis identified target sequences of the miR-29 family of miRNAs to be overrepresented in age-down-regulated genes. Concomitantly, expression microarray analysis in mouse kidney and lung showed that all members of the miR-29 family, miR-29a, -b, and -c, were strongly up-regulated from 1 to 6 weeks of age. Real-time PCR confirmed that miR-29a, -b, and -c were up-regulated with age in liver, kidney, lung, and heart, and their expression levels were higher in hepatocytes isolated from 5-week-old mice than in hepatocytes from embryonic mouse liver at embryonic day 16.5. We next focused on 3 predicted miR-29 target genes (Igf1, Imp1, and Mest), all of which are growth-promoting. A 3'-untranslated region containing the predicted target sequences from each gene was placed individually in a luciferase reporter construct. Transfection of miR-29 mimics suppressed luciferase gene activity for all 3 genes, and this suppression was diminished by mutating the target sequences, suggesting that these genes are indeed regulated by miR-29. Taken together, the findings suggest that up-regulation of miR-29 during juvenile life drives the down-regulation of multiple growth-promoting genes, thus contributing to physiological slowing and eventual cessation of body growth.

Indexed as

3' Untranslated RegionsAgingAnimalsAnimals, NewbornBase SequenceDown-RegulationGene Expression Regulation, DevelopmentalGrowth and DevelopmentHEK293 CellsHumansKidneyLungMaleMice, Inbred C57BLMicroRNAsMolecular Sequence Data3' Untranslated RegionsMicroRNAsMIRN29 microRNA, mouseRNA, Messenger

Identifiers

PMID25866874
PMCPMC4447640
OpenAlexW2106780617

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.