Evidence map›Paper›PMID 25866769›Full record

ArticleBioMed research international2015

Identification of the alternative splicing of the UL49 locus of human cytomegalovirus.

Guang Yang, Wei Li, Wenzhen Liao, Xin Zhang, Yi Zou, Jianfeng Dai, Yueqin Li, Chunxia Jing, Tianhong Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Guang YangDepartment of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China ; Department of Parasitology, Medical School, Jinan University, Guangzhou 510632, China.
Wei LiDepartment of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Wenzhen LiaoDepartment of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Xin ZhangDepartment of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Yi ZouDepartment of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Jianfeng DaiDepartment of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Yueqin LiDepartment of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Chunxia JingDepartment of Parasitology, Medical School, Jinan University, Guangzhou 510632, China.
Tianhong ZhouDepartment of Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The UL49 ORF of human cytomegalovirus (HCMV) is essential for viral replication; conserved among all herpes viruses; however, the function is unclear. Once the UL49 ORF was precisely deleted from the start to stop codon, the mutant did not yield infectious progeny. In this study, we find out many alternatively processed ESTs in UL49 locus in HCMV-infected cells, in which there are two novel transcription termination sites in UL49 locus. Most of these ESTs are rare transcripts that contain directed repeat sequences in the intron splicing regions. There is a typical GU-AG intron splicing site in UL49Y transcripts. The 1847 bp UL49Y cDNA spans an ORF from 335 to 1618 and encodes a putative protein of 427 amino acids with a predicted molecular mass of 47.1 kDa. All the new EST sequences and UL49Y cDNA sequence have been deposited in the GenBank database (GenBank Accession nos. GW314860-GW314900 and GU376796). This study provides us with very important clues for revealing the importance of the UL49 locus alternative splicing.

Indexed as

Viral ProteinsAlternative SplicingBase SequenceCytomegalovirusGene Expression Regulation, ViralGenetic LociHumansMolecular Sequence DataOpen Reading FramesUL49 protein, human cytomegalovirusViral Proteins

Identifiers

PMID25866769
PMCPMC4383306

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.