ArticleCellular and molecular bioengineering2015
Probing the biophysical properties of primary breast tumor-derived fibroblasts.
Article in Cellular and molecular bioengineering, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Physical Forces in Glioblastoma Migration: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Strain Promotes Triple Negative Breast Cancer Proliferation and Migration Via VEGFR-2.Cellular and molecular bioengineering · 2025Article
- Strain and hyaluronic acid interact to regulate ovarian cancer cell proliferation, migration, and drug resistance.Mechanobiology in medicine · 2024Article
- Biomechanical stimulation promotes blood vessel growth despite VEGFR-2 inhibition.BMC biology · 2023Article
- External mechanical loading overrules cell-cell mechanical communication in sprouting angiogenesis during early bone regeneration.PLoS computational biology · 2023Article
- Cancer cells can be killed mechanically or with combinations of cytoskeletal inhibitors.Frontiers in pharmacology · 2022Review
- RapidET: a MEMS-based platform for label-free and rapid demarcation of tumors from normal breast biopsy tissues.Microsystems & nanoengineering · 2022Article
- Mechanical Studies of the Third Dimension in Cancer: From 2D to 3D Model.International journal of molecular sciences · 2021Review
- Micro-strains in the extracellular matrix induce angiogenesis.Lab on a chip · 2020Article
- The Role of Microenvironmental Cues and Mechanical Loading Milieus in Breast Cancer Cell Progression and Metastasis.Frontiers in bioengineering and biotechnology · 2020Review
- Cancer-associated fibroblasts support vascular growth through mechanical force.Scientific reports · 2017Article
- Embedded Spheroids as Models of the Cancer Microenvironment.Advanced biosystems · 2017Article
- The Mechanics of Single Cell and Collective Migration of Tumor Cells.Journal of biomechanical engineering · 2017Review
- Review
- Microvesicles released from tumor cells disrupt epithelial cell morphology and contractility.Journal of biomechanics · 2016Article
- Extracellular Matrix Rigidity-dependent Sphingosine-1-phosphate Secretion Regulates Metastatic Cancer Cell Invasion and Adhesion.Scientific reports · 2016Article
- Comparative mechanisms of cancer cell migration through 3D matrix and physiological microtracks.American journal of physiology. Cell physiology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
As cancer progresses, cells must adapt to a new and stiffer environment, which can ultimately alter how normal cells within the tumor behave. In turn, these cells are known to further aid tumor progression. Therefore, there is potentially a unique avenue to better understand metastatic potential through single-cell biophysical assays performed on patient-derived cells. Here, we perform biophysical characterization of primary human fibroblastic cells obtained from mammary carcinoma and normal contralateral tissue. Through a series of tissue dissociation, differential centrifugation and trypsinization steps, we isolate an adherent fibroblastic population viable for biomechanical testing. 2D TFM and 3D migration measurements in a collagen matrix show that fibroblasts obtained from patient tumors generate more traction forces and display improved migration potential than their counterparts from normal tissue. Moreover, through the use of an embedded spheroid model, we confirmed the extracellular matrix (ECM) remodeling behavior of primary cells isolated from carcinoma. Overall, correlating biophysical characterization of normal- and carcinoma-derived samples from individual patient along with patient outcome may become a powerful approach to further our comprehension of metastasis and ultimately design drug targets on a patient-specific basis.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.