ArticleBiotechnology for biofuels2015
A microbial platform for renewable propane synthesis based on a fermentative butanol pathway.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 64 citations in OpenAlex.
- Beyond methane, new frontiers in anaerobic microbial hydrocarbon utilizing pathways.Microbial biotechnology · 2024Review
- Co-production of biofuel, bioplastics and biochemicals during extended fermentation of Halomonas bluephagenesis.Microbial biotechnology · 2023Article
- Biogenic propane production by a marineFrontiers in microbiology · 2022Article
- One-pot production of butyl butyrate from glucose using a cognate "diamond-shaped" E. coli consortium.Bioresources and bioprocessing · 2021Article
- Engineering nature for gaseous hydrocarbon production.Microbial cell factories · 2020Review
- In silico co-factor balance estimation using constraint-based modelling informs metabolic engineering in Escherichia coli.PLoS computational biology · 2020Article
- Article
- Promoter engineering for microbial bio-alkane gas production.Synthetic biology (Oxford, England) · 2020Article
- Renewable and tuneable bio-LPG blends derived from amino acids.Biotechnology for biofuels · 2020Article
- Addition of formate dehydrogenase increases the production of renewable alkane from an engineered metabolic pathway.The Journal of biological chemistry · 2019Article
- Identification of non-conserved residues essential for improving the hydrocarbon-producing activity of cyanobacterial aldehyde-deformylating oxygenase.Biotechnology for biofuels · 2019Article
- The influence of fatty acid supply and aldehyde reductase deletion on cyanobacteria alkane generating pathway in Escherichia coli.Journal of industrial microbiology & biotechnology · 2018Article
- Engineering metabolic pathways inSynthetic and systems biotechnology · 2018Article
- Comparison of orthologous cyanobacterial aldehyde deformylating oxygenases in the production of volatile C3-C7 alkanes in engineeredMetabolic engineering communications · 2017Article
- Holistic bioengineering: rewiring central metabolism for enhanced bioproduction.The Biochemical journal · 2017Review
- Effects of acetoacetyl-CoA synthase expression on production of farnesene in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2017Article
- Fueling the future with biomass: Processes and pathways for a sustainable supply of hydrocarbon fuels and biogas.Engineering in life sciences · 2017Review
- SYNBIOCHEM Synthetic Biology Research Centre, Manchester - A UK foundry for fine and speciality chemicals production.Synthetic and systems biotechnology · 2016Article
- SYNBIOCHEM-a SynBio foundry for the biosynthesis and sustainable production of fine and speciality chemicals.Biochemical Society transactions · 2016Article
- Engineered Production of Short Chain Fatty Acid in Escherichia coli Using Fatty Acid Synthesis Pathway.PloS one · 2016Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundPropane (C3H8) is a volatile hydrocarbon with highly favourable physicochemical properties as a fuel, in addition to existing global markets and infrastructure for storage, distribution and utilization in a wide range of applications. Consequently, propane is an attractive target product in research aimed at developing new renewable alternatives to complement currently used petroleum-derived fuels. This study focuses on the construction and evaluation of alternative microbial biosynthetic pathways for the production of renewable propane. The new pathways utilize CoA intermediates that are derived from clostridial-like fermentative butanol pathways and are therefore distinct from the first microbial propane pathways recently engineered in Escherichia coli.
resultsWe report the assembly and evaluation of four different synthetic pathways for the production of propane and butanol, designated a) atoB-adhE2 route, b) atoB-TPC7 route, c) nphT7-adhE2 route and d) nphT7-TPC7 route. The highest butanol titres were achieved with the atoB-adhE2 (473 ± 3 mg/L) and atoB-TPC7 (163 ± 2 mg/L) routes. When aldehyde deformylating oxygenase (ADO) was co-expressed with these pathways, the engineered hosts also produced propane. The atoB-TPC7-ADO pathway was the most effective in producing propane (220 ± 3 μg/L). By (i) deleting competing pathways, (ii) including a previously designed ADOA134F variant with an enhanced specificity towards short-chain substrates and (iii) including a ferredoxin-based electron supply system, the propane titre was increased (3.40 ± 0.19 mg/L).
conclusionsThis study expands the metabolic toolbox for renewable propane production and provides new insight and understanding for the development of next-generation biofuel platforms. In developing an alternative CoA-dependent fermentative butanol pathway, which includes an engineered ADO variant (ADOA134F), the study addresses known limitations, including the low bio-availability of butyraldehyde precursors and poor activity of ADO with butyraldehyde. Graphical abstractPropane synthesis derived from a fermentative butanol pathway is enabled by metabolic engineering.
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