ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2015
Evaluation of DNA extraction methods for the detection of Cytomegalovirus in dried blood spots.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Detection of Viral Nucleic Acid in Specimens Spotted on Commercial Filter Papers: A Review and Meta-Analysis.Viruses · 2026Pooled it
- Review
- Cross-tissue comparison of epigenetic aging clocks in humans.Aging cell · 2025Article
- Comparison and Optimization of DNA Extraction Methods for Human DNA from Dried Blood Spot Samples.Pediatric reports · 2025Article
- Comparison of the recovery of PCR-detectable porcine epidemic diarrhea virus and porcine reproductive and respiratory syndrome virus from filter papers under laboratory conditions.Frontiers in cellular and infection microbiology · 2025Article
- Maternal and congenital human cytomegalovirus infection: laboratory testing for detection and diagnosis.Journal of clinical microbiology · 2024Review
- Implementation of pooled saliva tests for universal screening of cCMV infection.Nature medicine · 2024Article
- Diagnostic utility of exome sequencing followed by research reanalysis in human brain malformations.Brain communications · 2024Article
- Cross-tissue comparison of telomere length and quality metrics of DNA among individuals aged 8 to 70 years.PloS one · 2024Article
- Study of diversity of mineral-forming bacteria in sabkha mats and sediments of mangrove forest in Qatar.Biotechnology reports (Amsterdam, Netherlands) · 2023Article
- Dried blood spots for Streptococcus pneumoniae and Haemophilus influenzae detection and serotyping among children < 5 years old in rural Mozambique.BMC pediatrics · 2020Article
- Diagnosing congenital Cytomegalovirus infection: don't get rid of dried blood spots.BMC infectious diseases · 2020Review
- Clinical Diagnostic Testing for Human Cytomegalovirus Infections.The Journal of infectious diseases · 2020Review
- Article
- Droplet Digital PCR for Estimating Absolute Abundances of Widespread Pelagibacter Viruses.Frontiers in microbiology · 2019Article
- Viral Loads in Congenital Cytomegalovirus Infection From a Highly Immune Population.Journal of the Pediatric Infectious Diseases Society · 2018Article
- Potential Benefit of Selective CMV Testing after Failed Newborn Hearing Screening.International journal of neonatal screening · 2018Review
- Cohort study on maternal cytomegalovirus seroprevalence and prevalence and clinical manifestations of congenital infection in China.Medicine · 2017Article
- Screening for Congenital Cytomegalovirus After Newborn Hearing Screening: What Comes Next?Pediatrics · 2017Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDried blood spots (DBS) are collected universally from newborns and may be valuable for the diagnosis of congenital Cytomegalovirus (CMV) infection. The reported analytical sensitivity for DBS testing compared to urine or saliva varies greatly across CMV studies. The purpose of this study was to directly compare the performance of various DNA extraction methods for identification of CMV in DBS including those used most often in CMV studies. STUDY
designWhatman(®) Grade 903 filter paper cards were spotted with blood samples from 25 organ transplant recipients who had confirmed CMV viremia. Six DNA extraction methods were compared for relative yield of viral and cellular DNA: 2 manual solution-based methods (Gentra Puregene, thermal shock), 2 manual silica column-based methods (QIAamp DNA Mini, QIAamp DNA Investigator), and 2 automated methods (M48 MagAttract Mini, QIAcube Investigator). DBS extractions were performed in triplicate followed by real-time quantitative PCR (qPCR).
resultsFor extraction of both viral and cellular DNA, two methods (QIAamp DNA Investigator and thermal shock) consistently gave the highest yields, and two methods (M48 MagAttract Mini and QIAamp DNA Mini) consistently gave the lowest yields. There was an average 3-fold difference in DNA yield between the highest and lowest yield methods.
conclusionThe choice of DNA extraction method is a major factor in the ability to detect low levels of CMV in DBS and can largely account for the wide range of DBS sensitivities reported in studies to date.
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