ArticleStructure (London, England : 1993)2015
FlaF Is a β-Sandwich Protein that Anchors the Archaellum in the Archaeal Cell Envelope by Binding the S-Layer Protein.
Article in Structure (London, England : 1993), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 69 citations in OpenAlex.
- Review
- Sulfolobales: Acidothermophilic archaea as models for biology and biotechnological applications.Engineering microbiology · 2026Review
- A multilayered cell envelope of a member of the Chloroflexota offers an anchoring platform for the archaellum.Frontiers in microbiology · 2026Article
- Structure of a functional archaellum in Bacteria of the Chloroflexota phylum.Nature microbiology · 2025Article
- How Does the Archaellum Work?Biomolecules · 2025Review
- Unusual cell surfaces, pili, and archaella of Thermoplasmatales archaea.The ISME journal · 2025Article
- Towards a molecular picture of the archaeal cell surface.Nature communications · 2024Article
- Involvement of ArlI, ArlJ, and CirA in archaeal type IV pilin-mediated motility regulation.Journal of bacteriology · 2024Article
- Involvement of ArlI, ArlJ, and CirA in Archaeal Type-IV Pilin-Mediated Motility Regulation.bioRxiv : the preprint server for biology · 2024Article
- Membrane lipid and expression responses ofFrontiers in microbiology · 2023Article
- Direct Observation of Archaellar Motor Rotation by Single-Molecular Imaging Techniques.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The cell biology of archaea.Nature microbiology · 2022Review
- A Micrarchaeon Isolate Is Covered by a Proteinaceous S-Layer.Applied and environmental microbiology · 2022Article
- An archaellum filament composed of two alternating subunits.Nature communications · 2022Article
- Towards Elucidating the Rotary Mechanism of the Archaellum Machinery.Frontiers in microbiology · 2022Review
- A comprehensive history of motility and Archaellation in Archaea.FEMS microbes · 2021Article
- How Did the Archaellum Get Its Rotation?Frontiers in microbiology · 2021Review
- Evolution of Archaellum Rotation Involved Invention of a Stator Complex by Duplicating and Modifying a Core Component.Frontiers in microbiology · 2021Article
- Motile ghosts of the halophilic archaeon,Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Multidomain ribosomal protein trees and the planctobacterial origin of neomura (eukaryotes, archaebacteria).Protoplasma · 2020Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Archaea employ the archaellum, a type IV pilus-like nanomachine, for swimming motility. In the crenarchaeon Sulfolobus acidocaldarius, the archaellum consists of seven proteins: FlaB/X/G/F/H/I/J. FlaF is conserved and essential for archaellum assembly but no FlaF structures exist. Here, we truncated the FlaF N terminus and solved 1.5-Å and 1.65-Å resolution crystal structures of this monotopic membrane protein. Structures revealed an N-terminal α-helix and an eight-strand β-sandwich, immunoglobulin-like fold with striking similarity to S-layer proteins. Crystal structures, X-ray scattering, and mutational analyses suggest dimer assembly is needed for in vivo function. The sole cell envelope component of S. acidocaldarius is a paracrystalline S-layer, and FlaF specifically bound to S-layer protein, suggesting that its interaction domain is located in the pseudoperiplasm with its N-terminal helix in the membrane. From these data, FlaF may act as the previously unknown archaellum stator protein that anchors the rotating archaellum to the archaeal cell envelope.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.