ArticleThe Journal of physiology2015
Astrocyte VAMP3 vesicles undergo Ca2+ -independent cycling and modulate glutamate transporter trafficking.
Article in The Journal of physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
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- The Impact of Small Molecule on the Astrocyte's Viability Derived From Epileptic Brain Tissues.Basic and clinical neuroscience · 2025Article
- Recycling of endocytic BDNF through extracellular vesicles in astrocytes.Scientific reports · 2025Article
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- Hippocampal astrocytes induce sex-dimorphic effects on memory.Cell reports · 2024Article
- Circular RNA Tmcc1 improves astrocytic glutamate metabolism and spatial memory via NF-κB and CREB signaling in a bile duct ligation mouse model: transcriptional and cellular analyses.Journal of neuroinflammation · 2023Article
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- Endocytic BDNF secretion regulated by Vamp3 in astrocytes.Scientific reports · 2021Article
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- Astrocytes respond to a neurotoxic Aβ fragment with state-dependent CaActa neuropathologica communications · 2021Article
- Lysosomal Functions in Glia Associated with Neurodegeneration.Biomolecules · 2021Review
- Human neurons from Christianson syndrome iPSCs reveal mutation-specific responses to rescue strategies.Science translational medicine · 2021Article
- Astrocytic cAMP modulates memory via synaptic plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The frequency-dependent effect of electrical fields on the mobility of intracellular vesicles in astrocytes.Biochemical and biophysical research communications · 2021Article
- Disruption of Glutamate Transport and Homeostasis by Acute Metabolic Stress.Frontiers in cellular neuroscience · 2021Article
- Are glutamate transporters neuroprotective or neurodegenerative during cerebral ischemia?Journal of molecular medicine (Berlin, Germany) · 2019Review
- The Astrocytic cAMP Pathway in Health and Disease.International journal of molecular sciences · 2019Review
- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely Tethered Ligands.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Gliotransmission: Beyond Black-and-White.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Review
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key pointsMouse cortical astrocytes express VAMP3 but not VAMP2. VAMP3 vesicles undergo Ca(2+) -independent exo- and endocytotic cycling at the plasma membrane. VAMP3 vesicle traffic regulates the recycling of plasma membrane glutamate transporters. cAMP modulates VAMP3 vesicle cycling and glutamate uptake. ABSTRACT: Previous studies suggest that small synaptic-like vesicles in astrocytes carry vesicle-associated vSNARE proteins, VAMP3 (cellubrevin) and VAMP2 (synaptobrevin 2), both contributing to the Ca(2+) -regulated exocytosis of gliotransmitters, thereby modulating brain information processing. Here, using cortical astrocytes taken from VAMP2 and VAMP3 knock-out mice, we find that astrocytes express only VAMP3. The morphology and function of VAMP3 vesicles were studied in cultured astrocytes at single vesicle level with stimulated emission depletion (STED) and total internal reflection fluorescence (TIRF) microscopies. We show that VAMP3 antibodies label small diameter (∼80 nm) vesicles and that VAMP3 vesicles undergo Ca(2+) -independent exo-endocytosis. We also show that this pathway modulates the surface expression of plasma membrane glutamate transporters and the glutamate uptake by astrocytes. Finally, using pharmacological and optogenetic tools, we provide evidence suggesting that the cytosolic cAMP level influences astrocytic VAMP3 vesicle trafficking and glutamate transport. Our results suggest a new role for VAMP3 vesicles in astrocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.