ArticleScientific reports2015
Defining minimum essential factors to derive highly pure human endothelial cells from iPS/ES cells in an animal substance-free system.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
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- Vascularization of Engineered Spatially Patterned Myocardial Tissue Derived From Human Pluripotent Stem CellsFrontiers in bioengineering and biotechnology · 2019Article
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- Global phenotypic characterisation of human platelet lysate expanded MSCs by high-throughput flow cytometry.Scientific reports · 2018Article
- Induced Pluripotent Stem Cell-Derived Endothelial Cells in Insulin Resistance and Metabolic Syndrome.Arteriosclerosis, thrombosis, and vascular biology · 2017Review
- Microfibrous Scaffolds Enhance Endothelial Differentiation and Organization of Induced Pluripotent Stem Cells.Cellular and molecular bioengineering · 2017Article
- Compliant substratum guides endothelial commitment from human pluripotent stem cells.Science advances · 2017Article
- Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium.BMC biology · 2017Article
- Optimizing mesoderm progenitor selection and three-dimensional microniche culture allows highly efficient endothelial differentiation and ischemic tissue repair from human pluripotent stem cells.Stem cell research & therapy · 2017Article
- Epigenetics in the Vascular Endothelium: Looking From a Different Perspective in the Epigenomics Era.Arteriosclerosis, thrombosis, and vascular biology · 2015Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is desirable to obtain unlimited supplies of endothelial cells for research and therapeutics. However, current methods of deriving endothelial cells from humans suffer from issues, such as limited supplies, contamination from animal substances, and lengthy/complicated procedures. In this article we developed a way to differentiate human iPS and ES cells to highly pure endothelial cells in 5 days. The chemically defined system is robust, easy to perform, and free of animal substances. Using the system, we verified that combined TGFβ and canonical Wnt agonists are essential and sufficient for iPS/ES cell-to-mesoderm transition. Besides, VEGF-KDR signaling alone is required for endothelial formation at high density while supplementation with FGF allows for colonial endothelial differentiation. Finally, anti-adsorptive agents could enrich the endothelial output by allowing selective attachment of the endothelial precursors. The system was validated to work on multiple iPS/ES cells lines to produce endothelial cells capable of forming capillary-like structures in vitro and integrating into host vasculature in vivo. In sum, the simple yet robust differentiation system permits the unlimited supply of human endothelial cells. The defined and animal substance-free nature of the system is compatible with clinical applications and characterization of endothelial differentiation in an unbiased manner.
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