ArticleStem cell research2015
Assessing reproductive toxicity of two environmental toxicants with a novel in vitro human spermatogenic model.
Article in Stem cell research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Naproxen and Ibuprofen Exposure Alter the Blood-Testis Barrier in a Novel In Vitro Model.International journal of molecular sciences · 2026Article
- Atrazine Induces Reproductive Toxicity in anBiomedicines · 2025Article
- Rapid identification of reproductive toxicants among environmental chemicals using an in vivo evaluation of gametogenesis in budding yeast Saccharomyces cerevisiae.Reproductive toxicology (Elmsford, N.Y.) · 2024Article
- Variation of sperm quality and circular RNA content in men exposed to environmental contamination with heavy metals in 'Land of Fires', Italy.Human reproduction (Oxford, England) · 2024Article
- Redox mechanisms of environmental toxicants on male reproductive function.Frontiers in cell and developmental biology · 2024Review
- Inheritance of paternal lifestyles and exposures through sperm DNA methylation.Nature reviews. Urology · 2023Review
- Suppression of trinucleotide repeat expansion in spermatogenic cells in Huntington's disease.Journal of assisted reproduction and genetics · 2022Article
- Implications of testicular ACE2 and the renin-angiotensin system for SARS-CoV-2 on testis function.Nature reviews. Urology · 2022Review
- Blastocyst development after fertilization with in vitro spermatids derived from nonhuman primate embryonic stem cells.F&S science · 2021Article
- Comparative efficacy of Gum Arabic (Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2020Article
- Detrimental effects of flame retardant, PBB153, exposure on sperm and future generations.Scientific reports · 2020Article
- BCL-2 Inhibitor Venetoclax Induces Autophagy-Associated Cell Death, Cell Cycle Arrest, and Apoptosis in Human Breast Cancer Cells.OncoTargets and therapy · 2020Article
- Reproductive toxic agents in work environments and related cases in Korea.Yeungnam University journal of medicine · 2020Article
- Per- and polyfluoroalkyl substances impact human spermatogenesis in a stem-cell-derived model.Systems biology in reproductive medicine · 2018Article
- Ubiquitous Flame-Retardant Toxicants Impair Spermatogenesis in a Human Stem Cell Model.iScience · 2018Article
- Environmentally toxicant exposures induced intragenerational transmission of liver abnormalities in mice.Open veterinary journal · 2017Article
- Retracted article: In vitro derivation of mammalian germ cells from stem cells and their potential therapeutic application.Cellular and molecular life sciences : CMLS · 2015Article
- Cannabis alters DNA methylation at maternally imprinted and autism candidate genes in spermatogenic cells.Systems biology in reproductive medicineArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Environmental influences and insults by reproductive toxicant exposure can lead to impaired spermatogenesis or infertility. Understanding how toxicants disrupt spermatogenesis is critical for determining how environmental factors contribute to impaired fertility. While current animal models are available, understanding of the reproductive toxic effects on human fertility requires a more robust model system. We recently demonstrated that human pluripotent stem cells can differentiate into spermatogonial stem cells/spermatogonia, primary and secondary spermatocytes, and haploid spermatids; a model that mimics many aspects of human spermatogenesis. Here, using this model system, we examine the effects of 2-bromopropane (2-BP) and 1,2,dibromo-3-chloropropane (DBCP) on in vitro human spermatogenesis. 2-BP and DBCP are non-endocrine disrupting toxicants that are known to impact male fertility. We show that acute treatment with either 2-BP or DBCP induces a reduction in germ cell viability through apoptosis. 2-BP and DBCP affect viability of different cell populations as 2-BP primarily reduces spermatocyte viability, whereas DBCP exerts a much greater effect on spermatogonia. Acute treatment with 2-BP or DBCP also reduces the percentage of haploid spermatids. Both 2-BP and DBCP induce reactive oxygen species (ROS) formation leading to an oxidized cellular environment. Taken together, these results suggest that acute exposure with 2-BP or DBCP causes human germ cell death in vitro by inducing ROS formation. This system represents a unique platform for assessing human reproductive toxicity potential of various environmental toxicants in a rapid, efficient, and unbiased format.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.