ArticlePlant physiology2015
Biphenyl 4-Hydroxylases Involved in Aucuparin Biosynthesis in Rowan and Apple Are Cytochrome P450 736A Proteins.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Biochemical characterization of benzaldehyde dehydrogenases from petunia.Plant molecular biology · 2025Article
- Independent evolution of plant natural products: Formation of benzoxazinoids in Consolida orientalis (Ranunculaceae).The Journal of biological chemistry · 2025Article
- Biphenyls and dibenzofurans of the rosaceous subtribe Malinae and their role as phytoalexins.Planta · 2023Review
- Biotransformation of Bisphenol by Human Cytochrome P450 2C9 Enzymes: A Density Functional Theory Study.Inorganic chemistry · 2023Article
- Formulated hydroxy fatty acids from fruit pomaces reduce apple scab development caused byFrontiers in plant science · 2023Article
- Comprehensive Analysis of Cytochrome P450 Monooxygenases Reveals Insight Into Their Role in Partial Resistance AgainstFrontiers in plant science · 2022Article
- Genome-wide identification and expression profiling of durian CYPome related to fruit ripening.PloS one · 2021Article
- Benzoate-CoA ligase contributes to the biosynthesis of biphenyl phytoalexins in elicitor-treated pear cell cultures.Plant cell reports · 2020Article
- Article
- Genes Involved in Stress Response and Especially in Phytoalexin Biosynthesis Are Upregulated in FourFrontiers in plant science · 2019Article
- The Genome Sequence of the Wild TomatoFrontiers in plant science · 2018Article
- Molecular architectures of benzoic acid-specific type III polyketide synthases.Acta crystallographica. Section D, Structural biology · 2017Article
- Transcriptomic analysis of molecular responses in Malus domestica 'M26' roots affected by apple replant disease.Plant molecular biology · 2017Article
- Expression of Biphenyl Synthase Genes and Formation of Phytoalexin Compounds in Three Fire Blight-Infected Pyrus communis Cultivars.PloS one · 2016Article
- Molecular Cloning and Characterization of a Xanthone Prenyltransferase from Hypericum calycinum Cell Cultures.Molecules (Basel, Switzerland) · 2015Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Upon pathogen attack, fruit trees such as apple (Malus spp.) and pear (Pyrus spp.) accumulate biphenyl and dibenzofuran phytoalexins, with aucuparin as a major biphenyl compound. 4-Hydroxylation of the biphenyl scaffold, formed by biphenyl synthase (BIS), is catalyzed by a cytochrome P450 (CYP). The biphenyl 4-hydroxylase (B4H) coding sequence of rowan (Sorbus aucuparia) was isolated and functionally expressed in yeast (Saccharomyces cerevisiae). SaB4H was named CYP736A107. No catalytic function of CYP736 was known previously. SaB4H exhibited absolute specificity for 3-hydroxy-5-methoxybiphenyl. In rowan cell cultures treated with elicitor from the scab fungus, transient increases in the SaB4H, SaBIS, and phenylalanine ammonia lyase transcript levels preceded phytoalexin accumulation. Transient expression of a carboxyl-terminal reporter gene construct directed SaB4H to the endoplasmic reticulum. A construct lacking the amino-terminal leader and transmembrane domain caused cytoplasmic localization. Functional B4H coding sequences were also isolated from two apple (Malus × domestica) cultivars. The MdB4Hs were named CYP736A163. When stems of cv Golden Delicious were infected with the fire blight bacterium, highest MdB4H transcript levels were observed in the transition zone. In a phylogenetic tree, the three B4Hs were closest to coniferaldehyde 5-hydroxylases involved in lignin biosynthesis, suggesting a common ancestor. Coniferaldehyde and related compounds were not converted by SaB4H.
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