Evidence map›Paper›PMID 25862163›Full record

ArticleSmall GTPases2015

Purification of nuclear localization signal-containing proteins and its application to investigation of the mechanisms of the cell division cycle.

Andri Christodoulou, Hideki Yokoyama

Open access · hybridAbstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Small GTPases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 95% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 3 countries.

Andri Christodouloua European Molecular Biology Laboratory ; Heidelberg , Germany.
Hideki Yokoyama
European Molecular Biology Laboratory · DEFriedrich Miescher Laboratory · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The GTP bound form of the Ran GTPase (RanGTP) in the nucleus promotes nuclear import of the proteins bearing nuclear localization signals (NLS). When nuclear envelopes break down during mitosis, RanGTP is locally produced around chromosomes and drives the assembly of the spindle early in mitosis and the nuclear envelope (NE) later. RanGTP binds to the heterodimeric nuclear transport receptor importin α/β and releases NLS proteins from the receptor. Liberated NLS proteins around chromosomes have been shown to play distinct, essential roles in spindle and NE assembly. Here we provide a highly specific protocol to purify NLS proteins from crude cell lysates. The pure NLS fraction is an excellent resource to investigate the NLS protein function and identify new mitotic regulators, uncovering fundamental mechanisms of the cell division cycle. It takes 2-3 days to obtain the NLS fraction.

Indexed as

MitosisNuclear Localization SignalsAnimalsCell Cycle ProteinsCell FractionationCell NucleusHeLa CellsHumansNuclear ProteinsOvumProtein Transportran GTP-Binding ProteinXenopusCell Cycle ProteinsNuclear Localization SignalsNuclear Proteinsran GTP-Binding ProteinCSF, cytostatic factorimportinNE, nuclear envelopeNLS, nuclear localization signalNLS proteinsNPC, nuclear pore complexnuclear envelope assemblynuclear transport receptorsRan GTPasespindle assemblyspindle maintenance

Identifiers

PMID25862163
PMCPMC4601338
OpenAlexW2082895400

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.