ArticleEvidence-based complementary and alternative medicine : eCAM2015
Antiproliferative and Apoptosis Induction Potential of the Methanolic Leaf Extract of Holarrhena floribunda (G. Don).
Article in Evidence-based complementary and alternative medicine : eCAM, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- UHPLC-QTOF-ESI-MS/MS, SNAP-MS Identification, In Silico Prediction of Pharmacokinetic Properties of Constituents from the Stem Bark ofBiomolecules · 2025Article
- Nature-Inspired Strategies in Cancer Management: The Potential of Plant Extracts in Modulating Tumour Biology.International journal of molecular sciences · 2025Review
- Jatonik polyherbal mixture induced rat liver MMPT pore opening in normal Wistar rat:Chinese herbal medicines · 2024Article
- Novel Collagen-Polyphenols-Loaded Silica Composites for Topical Application.Pharmaceutics · 2023Article
- Chloroxylon swietenia (Roxb.) DC induces cell death and apoptosis by down-regulating the NF-κB pathway in MCF-7 breast cancer cells: In vitro and in vivo investigations.Cancer reports (Hoboken, N.J.) · 2022Article
- In Vitro Cytotoxic Activity of African Plants: A Review.Molecules (Basel, Switzerland) · 2022Review
- Distinct Mechanisms of Cytotoxicity in Novel Nitrogenous Heterocycles: Future Directions for a New Anti-Cancer Agent.Molecules (Basel, Switzerland) · 2022Article
- Prophylactic or therapeutic administration of Holarrhena floribunda hydro ethanol extract suppresses complete Freund's adjuvant-induced arthritis in Sprague-Dawley rats.Journal of inflammation (London, England) · 2022Article
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- Article
- Antioxidant and Anticancer Efficacies ofBioMed research international · 2021Article
- Article
- Chemopreventive Effects and Antioxidant Capacity of Combined Leaf Extracts ofEvidence-based complementary and alternative medicine : eCAM · 2020Article
- Corrigendum to "Antiproliferative and Apoptosis Induction Potential of the Methanolic Leaf Extract ofEvidence-based complementary and alternative medicine : eCAM · 2020Article
- Cytotoxic and cell cycle arrest properties of two steroidal alkaloids isolated from Holarrhena floribunda (G. Don) T. Durand & Schinz leaves.BMC complementary and alternative medicine · 2019Article
- Greensporone A, a Fungal Secondary Metabolite Suppressed Constitutively Activated AKT via ROS Generation and Induced Apoptosis in Leukemic Cell Lines.Biomolecules · 2019Article
- Exosomes from Macrophages Exposed to Apoptotic Breast Cancer Cells Promote Breast Cancer Proliferation and Metastasis.Journal of Cancer · 2019Article
- Interplay between Endoplasmic Reticular Stress and Survivin in Colonic Epithelial Cells.Cells · 2018Article
- Crude Methanol Extract ofIranian journal of pharmaceutical research : IJPR · 2018Article
- Cytotoxic and Apoptotic Effects of Govaniadine Isolated fromBioMed research international · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural plant products with potent growth inhibition and apoptosis induction properties are extensively being investigated for their cancer chemopreventive potential. Holarrhena floribunda (HF) is used in a wide range of traditional medicine practices. The present study investigated the antiproliferative and apoptosis induction potential of methanolic leaf extracts of HF against breast (MCF-7), colorectal (HT-29), and cervical (HeLa) cancer cells relative to normal KMST-6 fibroblasts. The MTT assay in conjunction with the trypan blue dye exclusion and clonogenic assays were used to determine the effects of the extracts on the cells. Caspase activities were assayed with Caspase-Glo 3/7 and Caspase-9 kits. Apoptosis induction was monitored by flow cytometry using the APOPercentage and Annexin V-FITC kits. Reactive oxygen species (ROS) was measured using the fluorogenic molecular probe 5-(and-6)-chloromethyl-2',7'-dichlorofluorescein diacetate acetyl ester and cell cycle arrest was detected with propidium iodide. Dose-response analyses of the extract showed greater sensitivity in cancer cell lines than in fibroblast controls. Induction of apoptosis, ROS, and cell cycle arrest were time- and dose-dependent for the cancer cell lines studied. These findings provide a basis for further studies on the isolation, characterization, and mechanistic evaluation of the bioactive compounds responsible for the antiproliferative activity of the plant extract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.