ArticleThe journal of physical chemistry. B2015
Visualizing KcsA conformational changes upon ion binding by infrared spectroscopy and atomistic modeling.
Article in The journal of physical chemistry. B, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Extracting accurate infrared lineshapes from weak vibrational probes at low concentrations.MethodsX · 2023Article
- AIM: A Mapping Program for Infrared Spectroscopy of Proteins.Journal of chemical theory and computation · 2022Article
- Computational IR Spectroscopy of Insulin Dimer Structure and Conformational Heterogeneity.The journal of physical chemistry. B · 2021Article
- AspThe Journal of biological chemistry · 2020Article
- Arg302 governs the pKProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Site-specific ion occupation in the selectivity filter causes voltage-dependent gating in a viral KScientific reports · 2018Article
- Refinement of Peptide Conformational Ensembles by 2D IR Spectroscopy: Application to Ala‒Ala‒Ala.Biophysical journal · 2018Article
- Structural properties determining low KThe Journal of biological chemistry · 2018Article
- Ion-protein interactions of a potassium ion channel studied by attenuated total reflection Fourier transform infrared spectroscopy.Biophysical reviews · 2018Review
- Two-Dimensional Spectroscopy Is Being Used to Address Core Scientific Questions in Biology and Materials Science.The journal of physical chemistry. B · 2018Review
- Time-resolved measurements of an ion channel conformational change driven by a membrane phase transition.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Probing the Effects of Gating on the Ion Occupancy of the KJournal of the American Chemical Society · 2017Article
- External cavity-quantum cascade laser infrared spectroscopy for secondary structure analysis of proteins at low concentrations.Scientific reports · 2016Article
- Instantaneous ion configurations in the K+ ion channel selectivity filter revealed by 2D IR spectroscopy.Science (New York, N.Y.) · 2016Article
- Specific interactions between alkali metal cations and the KcsA channel studied using ATR-FTIR spectroscopy.Biophysics and physicobiology · 2015Article
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Authors and funding
6 authors.
Funding
Abstract
The effect of ion binding in the selectivity filter of the potassium channel KcsA is investigated by combining amide I Fourier-transform infrared spectroscopy with structure-based spectral modeling. Experimental difference IR spectra between K(+)-bound KcsA and Na(+)-bound KcsA are in good qualitative agreement with spectra modeled from structural ensembles generated from molecular dynamics simulations. The molecular origins of the vibrational modes contributing to differences in these spectra are determined not only from structural differences in the selectivity filter but also from the pore helices surrounding this region. Furthermore, the coordination of K(+) or Na(+) to carbonyls in the selectivity filter effectively decouples the vibrations of those carbonyls from the rest of the protein, creating local probes of the electrostatic environment. The results suggest that it is necessary to include the influence of the surrounding helices in discussing selectivity and transport in KcsA and, on a more general level, that IR spectroscopy offers a nonperturbative route to studying the structure and dynamics of ion channels.
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