ArticlePloS one2015
De novo transcriptome assembly and the putative biosynthetic pathway of steroidal sapogenins of Dioscorea composita.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 37 citations in OpenAlex.
- Transcriptome and metabolome reveal the accumulation of secondary metabolites in different species of Dioscorea.BMC plant biology · 2025Article
- Understory growth ofFrontiers in microbiology · 2024Article
- Dioscorea composita WRKY5 positively regulates AtSOD1 and AtABF2 to enhance drought and salt tolerances.Plant cell reports · 2023Article
- The Identification ofMolecules (Basel, Switzerland) · 2023Article
- Endophyte-inoculated rhizomes ofFrontiers in microbiology · 2023Article
- The genome ofHorticulture research · 2022Article
- Article
- A customized nuclear target enrichment approach for developing a phylogenomic baseline forApplications in plant sciences · 2019Article
- Review
- Identification, characterization and expression analysis of genes involved in steroidal saponin biosynthesis in Dracaena cambodiana.Journal of plant research · 2018Article
- De novo assembly and annotation of the Zhe-Maidong (Ophiopogon japonicus (L.f.) Ker-Gawl) transcriptome in different growth stages.Scientific reports · 2017Article
- Article
- Spatial transcriptome analysis provides insights of key gene(s) involved in steroidal saponin biosynthesis in medicinally important herb Trillium govanianum.Scientific reports · 2017Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant Dioscorea composita has important applications in the medical and energy industries, and can be used for the extraction of steroidal sapogenins (important raw materials for the synthesis of steroidal drugs) and bioethanol production. However, little is known at the genetic level about how sapogenins are biosynthesized in this plant. Using Illumina deep sequencing, 62,341 unigenes were obtained by assembling its transcriptome, and 27,720 unigenes were annotated. Of these, 8,022 unigenes were mapped to 243 specific pathways, and 531 unigenes were identified to be involved in 24 secondary metabolic pathways. 35 enzymes, which were encoded by 79 unigenes, were related to the biosynthesis of steroidal sapogenins in this transcriptome database, covering almost all the nodes in the steroidal pathway. The results of real-time PCR experiments on ten related transcripts (HMGR, MK, SQLE, FPPS, DXS, CAS, HMED, CYP51, DHCR7, and DHCR24) indicated that sapogenins were mainly biosynthesized by the mevalonate pathway. The expression of these ten transcripts in the tuber and leaves was found to be much higher than in the stem. Also, expression in the shoots was low. The nucleotide and protein sequences and conserved domains of four related genes (HMGR, CAS, SQS, and SMT1) were highly conserved between D. composita and D. zingiberensis; but expression of these four genes is greater in D. composita. However, there is no expression of these key enzymes in potato and no steroidal sapogenins are synthesized.
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