ArticleLipids2015
Alteration of Wax Ester Content and Composition in Euglena gracilis with Gene Silencing of 3-ketoacyl-CoA Thiolase Isozymes.
Article in Lipids, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Derlin-like proteins are essential for the function of Euglena gracilis chloroplasts.Plant physiology · 2026Article
- Transient molecular chimerism for exploiting xenogeneic organelles.Nature communications · 2026Article
- Engineering Wax Ester Composition in Euglena gracilis Using Genome Editing.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Metabolic network reconstruction ofFrontiers in microbiology · 2023Review
- Euglena International Network (EIN): Driving euglenoid biotechnology for the benefit of a challenged world.Biology open · 2022Article
- A Synthetic Biology Perspective on the Bioengineering Tools for an Industrial Microalga:Frontiers in bioengineering and biotechnology · 2022Review
- A strategic approach to apply bacterial substances for increasing metabolite productions of Euglena gracilis in the bioreactor.Applied microbiology and biotechnology · 2021Article
- Characterization of sulfur-compound metabolism underlying wax-ester fermentation in Euglena gracilis.Scientific reports · 2019Article
- Bioproducts FromFrontiers in bioengineering and biotechnology · 2019Review
- Comparative proteomic analysis of mitochondria isolated from Euglena gracilis under aerobic and hypoxic conditions.PloS one · 2019Article
- Anaerobic respiration coupled with mitochondrial fatty acid synthesis in wax ester fermentation by Euglena gracilis.FEBS letters · 2018Article
- De novo assembly and comparative transcriptome analysis of Euglena gracilis in response to anaerobic conditions.BMC genomics · 2016Article
- Engineering Anaerobic Metabolic Branch Points to Control Carbon Allocation Between Wax Ester Synthesis and Succinate Accumulation in Euglena gracilis.The Journal of eukaryotic microbiologyArticle
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Euglena gracilis produces wax ester under hypoxic and anaerobic culture conditions with a net synthesis of ATP. In wax ester fermentation, fatty acids are synthesized by reversing beta-oxidation in mitochondria. A major species of wax ester produced by E. gracilis is myristyl myristate (14:0-14:0Alc). Because of its shorter carbon chain length with saturated compounds, biodiesel produced from E. gracilis wax ester may have good cold flow properties with high oxidative stability. We reasoned that a slight metabolic modification would enable E. gracilis to produce a biofuel of ideal composition. In order to produce wax ester with shorter acyl chain length, we focused on isozymes of the enzyme 3-ketoacyl-CoA thiolase (KAT), a condensing enzyme of the mitochondrial fatty acid synthesis pathway in E. gracilis. We performed a gene silencing study of KAT isozymes in E. gracilis. Six KAT isozymes were identified in the E. gracilis EST database, and silencing any three of them (EgKAT1-3) altered the wax ester amount and composition. In particular, silencing EgKAT1 induced a significant compositional shift to shorter carbon chain lengths in wax ester. A model fuel mixture inferred from the composition of wax ester in EgKAT1-silenced cells showed a significant decrease in melting point compared to that of the control cells.
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