ArticlePloS one2015
In Ovo injection of betaine affects hepatic cholesterol metabolism through epigenetic gene regulation in newly hatched chicks.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 67 citations in OpenAlex.
- Effects of in ovo stimulation with potential epigenetic modulators on immune system phenotype across three generations in a chicken model.Scientific reports · 2025Article
- Green forage impacts on the DNA methylation in the ruminal wall of Italian mediterranean dairy buffaloes.Scientific reports · 2025Article
- In ovo betaine and heat acclimation affect hatching results, growth performance and immunity of the broilers under cyclic heat stress.Tropical animal health and production · 2024Article
- How Does Nutrition Affect the Epigenetic Changes in Dairy Cows?Animals : an open access journal from MDPI · 2023Review
- The effects of plant extracts on lipid metabolism of chickens - A review.Animal bioscience · 2023Article
- Maternal dietary methionine restriction alters hepatic expression of one-carbon metabolism and epigenetic mechanism genes in the ducklings.BMC genomics · 2022Article
- Betaine: A Potential Nutritional Metabolite in the Poultry Industry.Animals : an open access journal from MDPI · 2022Review
- Nutrient-Mediated Perception and Signalling in Human Metabolism: A Perspective of Nutrigenomics.International journal of molecular sciences · 2022Review
- Distinct Patterns of GR Transcriptional Regulation in Liver and Muscle of LPS-Challenged Weaning Piglets.International journal of molecular sciences · 2022Article
- Betaine Promotes Fat Accumulation and Reduces Injury in Landes Goose Hepatocytes by Regulating Multiple Lipid Metabolism Pathways.Animals : an open access journal from MDPI · 2022Article
- In ovo injection of betaine promotes adrenal steroidogenesis in pre-hatched chicken fetuses.Poultry science · 2022Article
- Maternal dietary methionine restriction alters the expression of energy metabolism genes in the duckling liver.BMC genomics · 2022Article
- Pre-hatching and post-hatching environmental factors related to epigenetic mechanisms in poultry.Journal of animal science · 2022Review
- Examining the extent of environmental contributions toward DNA methylation and phenotypic variation.Animal frontiers : the review magazine of animal agriculture · 2021Article
- Chicken embryo as a model in epigenetic research.Poultry science · 2021Review
- How Does Protein Nutrition Affect the Epigenetic Changes in Pig? A Review.Animals : an open access journal from MDPI · 2021Review
- Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes.Poultry science · 2020Article
- Dietary betaine improves egg-laying rate in hens through hypomethylation and glucocorticoid receptor-mediated activation of hepatic lipogenesis-related genes.Poultry science · 2020Article
- Article
- Dietary Betaine Addition Promotes Hepatic Cholesterol Synthesis, Bile Acid Conversion, and Export in Rats.Nutrients · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Betaine is reported to regulate hepatic cholesterol metabolism in mammals. Chicken eggs contain considerable amount of betaine, yet it remains unknown whether and how betaine in the egg affects hepatic cholesterol metabolism in chicks. In this study, eggs were injected with betaine at 2.5 mg/egg and the hepatic cholesterol metabolism was investigated in newly hatched chicks. Betaine did not affect body weight or liver weight, but significantly increased the serum concentration (P < 0.05) and the hepatic content (P < 0.01) of cholesterol. Accordingly, the cholesterol biosynthetic enzyme HMGCR was up-regulated (P < 0.05 for both mRNA and protein), while CYP7A1 which converts cholesterol to bile acids was down-regulated (P < 0.05 for mRNA and P = 0.07 for protein). Moreover, hepatic protein content of the sterol-regulatory element binding protein 1 which regulates cholesterol and lipid biosynthesis, and the mRNA abundance of ATP binding cassette sub-family A member 1 (ABCA1) which mediates cholesterol counter transport were significantly (P < 0.05) increased in betaine-treated chicks. Meanwhile, hepatic protein contents of DNA methyltransferases 1 and adenosylhomocysteinase-like 1 were increased (P < 0.05), which was associated with global genomic DNA hypermethylation (P < 0.05) and diminished gene repression mark histone H3 lysine 27 trimethylation (P < 0.05). Furthermore, CpG methylation level on gene promoters was found to be increased (P < 0.05) for CYP7A1 yet decreased (P < 0.05) for ABCA1. These results indicate that in ovo betaine injection regulates hepatic cholesterol metabolism in chicks through epigenetic mechanisms including DNA and histone methylations.
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