Evidence map›Paper›PMID 25858960›Full record

ArticleG3 (Bethesda, Md.)2015

BAC-End Sequence-Based SNP Mining in Allotetraploid Cotton (Gossypium) Utilizing Resequencing Data, Phylogenetic Inferences, and Perspectives for Genetic Mapping.

Amanda M Hulse-Kemp, Hamid Ashrafi, Kevin Stoffel, Xiuting Zheng, Christopher A Saski, Brian E Scheffler, David D Fang, Z Jeffrey Chen, Allen Van Deynze, David M Stelly

Abstract read
In one paragraph

Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Amanda M Hulse-KempDepartment of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843 Interdisciplinary Genetics Program, Texas A&M University, College Station, Texas 77843.ORCID http://orcid.org/0000-0001-9670-9433
Hamid AshrafiSeed Biotechnology Center, University of California, Davis, California 95616.ORCID http://orcid.org/0000-0002-2094-2367
Kevin StoffelSeed Biotechnology Center, University of California, Davis, California 95616.ORCID http://orcid.org/0000-0002-3468-4119
Xiuting ZhengDepartment of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843.ORCID http://orcid.org/0000-0002-8574-4708
Christopher A SaskiClemson University Genomics Institute, Clemson University, Clemson, South Carolina 29634.ORCID http://orcid.org/0000-0002-2780-4274
Brian E SchefflerUSDA-ARS, Genomics and Bioinformatics Research Unit, Stoneville, Mississippi 38766.ORCID http://orcid.org/0000-0003-1968-8952
David D FangUSDA-ARS, Cotton Fiber Bioscience Research Unit, New Orleans, Louisiana 70124.ORCID http://orcid.org/0000-0003-0036-5459
Z Jeffrey ChenDepartment of Molecular Biosciences, Center for Computational Biology and Bioinformatics, and Institute for Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712.
Allen Van DeynzeSeed Biotechnology Center, University of California, Davis, California 95616.ORCID http://orcid.org/0000-0002-2093-0577
David M StellyDepartment of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843 Interdisciplinary Genetics Program, Texas A&M University, College Station, Texas 77843 stelly@tamu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A bacterial artificial chromosome library and BAC-end sequences for cultivated cotton (Gossypium hirsutum L.) have recently been developed. This report presents genome-wide single nucleotide polymorphism (SNP) mining utilizing resequencing data with BAC-end sequences as a reference by alignment of 12 G. hirsutum L. lines, one G. barbadense L. line, and one G. longicalyx Hutch and Lee line. A total of 132,262 intraspecific SNPs have been developed for G. hirsutum, whereas 223,138 and 470,631 interspecific SNPs have been developed for G. barbadense and G. longicalyx, respectively. Using a set of interspecific SNPs, 11 randomly selected and 77 SNPs that are putatively associated with the homeologous chromosome pair 12 and 26, we mapped 77 SNPs into two linkage groups representing these chromosomes, spanning a total of 236.2 cM in an interspecific F2 population (G. barbadense 3-79 × G. hirsutum TM-1). The mapping results validated the approach for reliably producing large numbers of both intraspecific and interspecific SNPs aligned to BAC-ends. This will allow for future construction of high-density integrated physical and genetic maps for cotton and other complex polyploid genomes. The methods developed will allow for future Gossypium resequencing data to be automatically genotyped for identified SNPs along the BAC-end sequence reference for anchoring sequence assemblies and comparative studies.

Indexed as

PhylogenyPolyploidyBase SequenceChromosome MappingChromosomes, Artificial, BacterialCluster AnalysisComputer SimulationContig MappingData MiningDiploidyGenetic LinkageGenetic LociGenetic MarkersGenome, BacterialGossypiumHeterozygoteGenetic MarkersBAC-derived SNPscotton genomicsintraspecificresequencingSNP genotyping

Identifiers

PMID25858960
PMCPMC4478540

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.