ArticlePlant physiology2015
SOS2-LIKE PROTEIN KINASE5, an SNF1-RELATED PROTEIN KINASE3-Type Protein Kinase, Is Important for Abscisic Acid Responses in Arabidopsis through Phosphorylation of ABSCISIC ACID-INSENSITIVE5.
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Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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71 citing papers in PubMed, 135 citations in OpenAlex.
- CIPK11 Regulates Plant Adaptation to Nitrate Availability in Arabidopsis.Plants (Basel, Switzerland) · 2026Article
- Evolutionary Characteristics of the Rice SnRK2 Family and ABI5 Interaction Analysis Reveal Their Key Regulatory Role in Osmotic Stress Signaling.International journal of molecular sciences · 2026Article
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- ABI5-binding proteins are substrates of key components in the ABA core signaling pathway affecting seeds.Plant physiology · 2026Article
- The resveratrol monomer enhances resistance to bacterial pathogen through the activation of SA-NPR1 signaling pathway inFrontiers in plant science · 2026Article
- Abscisic acid regulates iron accumulation in plant seeds via the PYLs-bHLH IVc-YSLs module.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- CPK2 Enhances ABA Sensitivity in Seed Germination and Root Growth by Promoting ABA-InducedPlants (Basel, Switzerland) · 2025Article
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- Identification of F-box proteins in ABA- and GA-regulated seed germination: interaction of GASA1 signalling peptide and ABA-induced ubiquitination.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Article
- The Alpha/Beta-Hydrolase Fold Superfamily inInternational journal of molecular sciences · 2025Article
- Unveiling the molecular mechanism underlying PSKR-mediated amplification of the ABA signaling in Arabidopsis thaliana.Plant cell reports · 2025Article
- Nitrate attenuates abscisic acid signaling via NIN-LIKE PROTEIN8 in Arabidopsis seed germination.The Plant cell · 2025Article
- The transcriptional regulation of ArabidopsisPhysiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- The maize GSK3-like kinase ZmSK1 negatively regulates drought tolerance by phosphorylating the transcription factor ZmCPP2.The Plant cell · 2025Article
- Allelic variation of TaABI5-A4 significantly affects seed dormancy in bread wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- The U-box E3 ubiquitin ligase PUB35 negatively regulates ABA signaling through AFP1-mediated degradation of ABI5.The Plant cell · 2024Article
- Calmodulin-like protein CML15 interacts with PP2C46/65 to regulate papaya fruit ripening via integrating calcium, ABA and ethylene signals.Plant biotechnology journal · 2024Article
- The role of CBL-CIPK signaling in plant responses to biotic and abiotic stresses.Plant molecular biology · 2024Review
- Post-translational modifications: emerging directors of cell-fate decisions during endoplasmic reticulum stress in Arabidopsis thaliana.Biochemical Society transactions · 2024Review
- Natural Allelic Variations ofInternational journal of molecular sciences · 2024Article
11 more citing papers are in PubMed but not listed here.
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19 authors at 3 institutions in 2 countries.
Xiaona ZhouState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Hongmei HaoState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Yuguo ZhangState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Yili BaiState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Wenbo ZhuState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Yunxia QinState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Feifei YuanState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Feiyi ZhaoState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Mengyao WangState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Jingjiang HuState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Hong XuState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Aiguang GuoState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Huixian ZhaoState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Yang ZhaoState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Cuiling CaoState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Yongqing YangState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Karen S SchumakerState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Yan GuoState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.).
Chang Gen XieState Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University, Yangling, Shaanxi 712100, China (X.Z., H.H., Y.B., W.Z., F.Y., M.W., J.H., H.X., A.G., H.Z., C.C., C.G.X.);State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China (Yu.Z., Y.Q., F.Z., Ya.Z., Y.Y., Y.G.); andSchool of Plant Sciences, University of Arizona, Tucson, Arizona 85721 (K.S.S.) changen.xie@nwsuaf.edu.cn.
Northwest A&F University · CNChina Agricultural University · CNUniversity of Arizona · US
Funding
No grant is acknowledged in the PubMed record.
Abstract
Abscisic acid (ABA) plays an essential role in seed germination. In this study, we demonstrate that one SNF1-related protein kinase3-type protein kinase, SOS2-like protein kinase5 (PKS5), is involved in ABA signal transduction via the phosphorylation of an interacting protein, abscisic acid-insensitive5 (ABI5). We found that pks5-3 and pks5-4, two previously identified PKS5 superactive kinase mutants with point mutations in the PKS5 FISL/NAF (a conserved peptide that is necessary for interaction with SOS3 or SOS3-like calcium binding proteins) motif and the kinase domain, respectively, are hypersensitive to ABA during seed germination. PKS5 was found to interact with ABI5 in yeast (Saccharomyces cerevisiae), and this interaction was further confirmed in planta using bimolecular fluorescence complementation. Genetic studies revealed that ABI5 is epistatic to PKS5. PKS5 phosphorylates a serine (Ser) residue at position 42 in ABI5 and regulates ABA-responsive gene expression. This phosphorylation was induced by ABA in vivo and transactivated ABI5. Expression of ABI5, in which Ser-42 was mutated to alanine, could not fully rescue the ABA-insensitive phenotypes of the abi5-8 and pks5-4abi5-8 mutants. In contrast, mutating Ser-42 to aspartate rescued the ABA insensitivity of these mutants. These data demonstrate that PKS5-mediated phosphorylation of ABI5 at Ser-42 is critical for the ABA regulation of seed germination and gene expression in Arabidopsis (Arabidopsis thaliana).
Indexed as
Abscisic AcidArabidopsisArabidopsis ProteinsBasic-Leucine Zipper Transcription FactorsEpistasis, GeneticGene Expression Regulation, PlantGenes, PlantGerminationModels, BiologicalMutationPhosphorylationPhosphoserineProtein BindingProtein Serine-Threonine KinasesSeedsTranscriptional ActivationABI5 protein, ArabidopsisAbscisic AcidArabidopsis ProteinsBasic-Leucine Zipper Transcription FactorsPhosphoserineProtein Serine-Threonine KinasesSIP4 protein, Arabidopsis
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