ArticlePlant biotechnology journal2016
Bottlenecks in carotenoid biosynthesis and accumulation in rice endosperm are influenced by the precursor-product balance.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 155 citations in OpenAlex.
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- CRISPR/Cas9-mediated editing of carotenoid biosynthesis genes alters carotenoid concentrations in kiwifruit.BMC plant biology · 2025Article
- Metabolically engineered plant cell cultures as biofactories for the production of high-value carotenoids astaxanthin and canthaxanthin.Scientific reports · 2025Article
- ORANGE family proteins: multifunctional chaperones shaping plant carotenoid level, plastid development, stress tolerance, and more.Molecular horticulture · 2025Review
- Identifying molecular targets for modulating carotenoid accumulation in rice grains.Biochemistry and biophysics reports · 2024Article
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- Engineering cyanobacteria as a new platform for producing taxol precursors directly from carbon dioxide.Biotechnology for biofuels and bioproducts · 2024Article
- Activation of the native PHYTOENE SYNTHASE 1 promoter by modifying near-miss cis-acting elements induces carotenoid biosynthesis in embryogenic rice callus.Plant cell reports · 2024Article
- Integrative Metabolome and Transcriptome Analyses Provide Insights into Carotenoid Variation in Different-Colored Peppers.International journal of molecular sciences · 2023Article
- Quality trait improvement in horticultural crops: OMICS and modern biotechnological approaches.Molecular biology reports · 2023Review
- Integrative Analysis of Metabolome and Transcriptome Revealed Lutein Metabolism Contributed to Yellow Flower Formation inPlants (Basel, Switzerland) · 2023Article
- Genetic and molecular basis of carotenoid metabolism in cereals.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- A new biotechnology for in-planta gene editing and its application in promoting flavonoid biosynthesis in bamboo leaves.Plant methods · 2023Article
- Multilevel interactions between native and ectopic isoprenoid pathways affect global metabolism in rice.Transgenic research · 2022Article
- Color recycling: metabolization of apocarotenoid degradation products suggests carbon regeneration via primary metabolic pathways.Plant cell reports · 2022Article
- Plant carotenoids: recent advances and future perspectives.Molecular horticulture · 2022Review
- Assessment of Biofortification Approaches Used to Improve Micronutrient-Dense Plants That Are a Sustainable Solution to Combat Hidden Hunger.Journal of soil science and plant nutrition · 2022Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The profile of secondary metabolites in plants reflects the balance of biosynthesis, degradation and storage, including the availability of precursors and products that affect the metabolic equilibrium. We investigated the impact of the precursor-product balance on the carotenoid pathway in the endosperm of intact rice plants because this tissue does not normally accumulate carotenoids, allowing us to control each component of the pathway. We generated transgenic plants expressing the maize phytoene synthase gene (ZmPSY1) and the bacterial phytoene desaturase gene (PaCRTI), which are sufficient to produce β-carotene in the presence of endogenous lycopene β-cyclase. We combined this mini-pathway with the Arabidopsis thaliana genes AtDXS (encoding 1-deoxy-D-xylulose 5-phosphate synthase, which supplies metabolic precursors) or AtOR (the ORANGE gene, which promotes the formation of a metabolic sink). Analysis of the resulting transgenic plants suggested that the supply of isoprenoid precursors from the MEP pathway is one of the key factors limiting carotenoid accumulation in the endosperm and that the overexpression of AtOR increased the accumulation of carotenoids in part by up-regulating a series of endogenous carotenogenic genes. The identification of metabolic bottlenecks in the pathway will help to refine strategies for the creation of engineered plants with specific carotenoid profiles.
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