ArticlePloS one2015
Methionine biosynthesis is essential for infection in the rice blast fungus Magnaporthe oryzae.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 52 citations in OpenAlex.
- A Dual-Role Transcription Factor FpCys3 Couples Glucose Sensing to Methionine Biosynthesis and Virulence in Fusarium pseudograminearum.Plant, cell & environment · 2026Article
- Transcription Factor MaHMG, the High-Mobility Group Protein, Is Implicated in Conidiation Pattern Shift and Stress Tolerance inJournal of fungi (Basel, Switzerland) · 2025Article
- Non-chemical control of fungal pathogens in crops: a one-health perspective on strategies, mechanisms, and future directions.Frontiers in plant science · 2025Review
- Article
- Fungal L-Methionine Biosynthesis Pathway Enzymes and Their Applications in Various Scientific and Commercial Fields.Biomolecules · 2024Review
- Exploring the Impact of Solid-State Fermentation on Fava Bean Flour: A Comparative Study ofFoods (Basel, Switzerland) · 2024Article
- Multi-omics analysis revealed that the protein kinase MoKin1 affected the cellular response to endoplasmic reticulum stress in the rice blast fungus, Magnaporthe oryzae.BMC genomics · 2024Article
- Methionine biosynthetic genes and methionine sulfoxide reductase A are required for Rhizoctonia solani AG1-IA to cause sheath blight disease in rice.Microbial biotechnology · 2024Article
- Article
- The bZIP transcription factor BIP1 of the rice blast fungus is essential for infection and regulates a specific set of appressorium genes.PLoS pathogens · 2024Article
- Morphological and transcriptional analysis of Colletotrichum lindemuthianum race 7 during early stages of infection in common bean.Genetics and molecular biology · 2024Article
- Methionine biosynthesis enzyme MoMet2 is required for rice blast fungus pathogenicity by promoting virulence gene expression via reducing 5mC modification.PLoS genetics · 2023Article
- Phosphocholine cytidylyltransferase MoPct1 is crucial for vegetative growth, conidiation, and appressorium-mediated plant infection byFrontiers in microbiology · 2023Article
- Pyridoxine biosynthesis protein MoPdx1 affects the development and pathogenicity ofFrontiers in cellular and infection microbiology · 2023Article
- Identification of small molecules targeting homoserine acetyl transferase from Mycobacterium tuberculosis and Staphylococcus aureus.Scientific reports · 2022Article
- Adenylsuccinate Synthetase MoADE12 Plays Important Roles in the Development and Pathogenicity of the Rice Blast Fungus.Journal of fungi (Basel, Switzerland) · 2022Article
- Transcriptional Profiles of a Foliar Fungal Endophyte (mSystems · 2022Article
- A Case Study in Saudi Arabia: Biodiversity of Maize Seed-Borne Pathogenic Fungi in Relation to Biochemical, Physiological, and Molecular Characteristics.Plants (Basel, Switzerland) · 2022Article
- Aspartate Transaminase AST2 Involved in Sporulation and Necrotrophic Pathogenesis in the HemibiotrophsFrontiers in microbiology · 2022Article
- Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methionine is a sulfur amino acid standing at the crossroads of several biosynthetic pathways. In fungi, the last step of methionine biosynthesis is catalyzed by a cobalamine-independent methionine synthase (Met6, EC 2.1.1.14). In the present work, we studied the role of Met6 in the infection process of the rice blast fungus, Magnaporthe oryzae. To this end MET6 null mutants were obtained by targeted gene replacement. On minimum medium, MET6 null mutants were auxotrophic for methionine. Even when grown in presence of excess methionine, these mutants displayed developmental defects, such as reduced mycelium pigmentation, aerial hypha formation and sporulation. They also displayed characteristic metabolic signatures such as increased levels of cysteine, cystathionine, homocysteine, S-adenosylmethionine, S-adenosylhomocysteine while methionine and glutathione levels remained unchanged. These metabolic perturbations were associated with the over-expression of MgCBS1 involved in the reversed transsulfuration pathway that metabolizes homocysteine into cysteine and MgSAM1 and MgSAHH1 involved in the methyl cycle. This suggests a physiological adaptation of M. oryzae to metabolic defects induced by the loss of Met6, in particular an increase in homocysteine levels. Pathogenicity assays showed that MET6 null mutants were non-pathogenic on both barley and rice leaves. These mutants were defective in appressorium-mediated penetration and invasive infectious growth. These pathogenicity defects were rescued by addition of exogenous methionine and S-methylmethionine. These results show that M. oryzae cannot assimilate sufficient methionine from plant tissues and must synthesize this amino acid de novo to fulfill its sulfur amino acid requirement during infection.
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