ArticleNature protocols2015
One-hour proteome analysis in yeast.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed.
- Atlas of lysine acetylation in the mouse.bioRxiv : the preprint server for biology · 2026Article
- High Dynamic Range Peptide Mass Spectrometry Using Segmented Precursor Ion Accumulation.Analytical chemistry · 2025Article
- The One Hour Human Proteome.Molecular & cellular proteomics : MCP · 2024Article
- Plasma/Serum Proteomics based on Mass Spectrometry.Protein and peptide letters · 2024Review
- Global detection of human variants and isoforms by deep proteome sequencing.Nature biotechnology · 2023Article
- HIV-1 virological synapse formation enhances infection spread by dysregulating Aurora Kinase B.PLoS pathogens · 2023Article
- Proteome-wide structural changes measured with limited proteolysis-mass spectrometry: an advanced protocol for high-throughput applications.Nature protocols · 2023Review
- Simple, efficient and thorough shotgun proteomic analysis with PatternLab V.Nature protocols · 2022Review
- Practical Effects of Intramolecular Hydrogen Rearrangement in Electron Transfer Dissociation-Based Proteomics.Journal of the American Society for Mass Spectrometry · 2022Article
- Lagovirus Non-structural Protein p23: A Putative Viroporin That Interacts With Heat Shock Proteins and Uses a Disulfide Bond for Dimerization.Frontiers in microbiology · 2022Article
- Mutant Presenilin 1 Dysregulates Exosomal Proteome Cargo Produced by Human-Induced Pluripotent Stem Cell Neurons.ACS omega · 2021Article
- DIA-based systems biology approach unveils E3 ubiquitin ligase-dependent responses to a metabolic shift.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Survival Following Traumatic Brain Injury inGenetics · 2020Article
- Proteomics in Non-model Organisms: A New Analytical Frontier.Journal of proteome research · 2020Article
- Article
- Orthogonal Proteomic Platforms and Their Implications for the Stable Classification of High-Grade Serous Ovarian Cancer Subtypes.iScience · 2020Article
- Dysregulation of Exosome Cargo by Mutant Tau Expressed in Human-induced Pluripotent Stem Cell (iPSC) Neurons Revealed by Proteomics Analyses.Molecular & cellular proteomics : MCP · 2020Article
- Discovery of Missing Methylation Sites on Endogenous Peptides of Human Cell Lines.Journal of the American Society for Mass Spectrometry · 2019Article
- Maximizing Tandem Mass Spectrometry Acquisition Rates for Shotgun Proteomics.Analytical chemistry · 2019Article
- Advanced Proteomics as a Powerful Tool for Studying Toxins of Human Bacterial Pathogens.Toxins · 2019Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Recent advances in chromatography and mass spectrometry (MS) have made rapid and deep proteomic profiling possible. To maximize the performance of the recently produced Orbitrap hybrid mass spectrometer, we have developed a protocol that combines improved sample preparation (including optimized cellular lysis by extensive bead beating) and chromatographic conditions (specifically, 30-cm capillary columns packed with 1.7-μm bridged ethylene hybrid material) and the manufacture of a column heater (to accommodate flow rates of 350-375 nl/min) that increases the number of proteins identified across a single liquid chromatography-tandem MS (LC-MS/MS) separation, thereby reducing the need for extensive sample fractionation. This strategy allowed the identification of up to 4,002 proteins (at a 1% false discovery rate (FDR)) in yeast (Saccharomyces cerevisiae strain BY4741) over 70 min of LC-MS/MS analysis. Quintuplicate analysis of technical replicates reveals 83% overlap at the protein level, thus demonstrating the reproducibility of this procedure. This protocol, which includes cell lysis, overnight tryptic digestion, sample analysis and database searching, takes ∼24 h to complete. Aspects of this protocol, including chromatographic separation and instrument parameters, can be adapted for the optimal analysis of other organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.