ArticlePloS one2015
Effect of vitrification on the microRNA transcriptome in mouse blastocysts.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Review
- Impact of third cleavage timing on blastulation and miRNA-429 expression in cryopreserved mouse embryos.Clinical and experimental reproductive medicine · 2026Article
- Transcriptomic Analysis of Vitrified-Warmed vs. Fresh Mouse Blastocysts: Cryo-Induced Physiological Mechanisms and Implantation Impact.International journal of molecular sciences · 2024Article
- Safety of embryo cryopreservation: insights from mid-term placental transcriptional changes.Reproductive biology and endocrinology : RB&E · 2024Article
- The gestational age-specific difference in birthweight between singletons born after fresh and frozen embryo transfer: A cohort study.Acta obstetricia et gynecologica Scandinavica · 2023Article
- Update on the Epigenomic Implication of Embryo Cryopreservation Methods Applied in Assisted Reproductive Technologies With Potential Long-Term Health Effects.Frontiers in cell and developmental biology · 2022Review
- Characterization of microRNAs in spent culture medium associated with human embryo quality and development.Annals of translational medicine · 2021Article
- Alterations of miR-16, miR-let-7a and their target genes expression in human blastocysts following vitrification and re-vitrification.Reproductive biology and endocrinology : RB&E · 2021Article
- Influence of hydrogel encapsulation during cryopreservation of ovarian tissues and impact of post-thawing in vitro culture systems in a research animal model.Clinical and experimental reproductive medicine · 2021Article
- Extracellular microRNAs: key players to explore the outcomes of in vitro fertilization.Reproductive biology and endocrinology : RB&E · 2021Article
- Effect of vitrification on biogenesis pathway and expression of development-related microRNAs in preimplantation mouse embryos.Cell and tissue banking · 2021Article
- Effects of Vitrification on the Blastocyst Gene Expression Profile in a Porcine Model.International journal of molecular sciences · 2021Article
- Prolonged Cryopreservation Negatively Affects Embryo Transfer Outcomes Following the Elective Freeze-All Strategy: A Multicenter Retrospective Study.Frontiers in endocrinology · 2021Article
- Health outcomes for Massachusetts infants after fresh versus frozen embryo transfer.Fertility and sterility · 2019Article
- Embryo cryopreservation and preeclampsia risk.Fertility and sterility · 2017Article
- Vitrification of Rhesus Macaque Mesenchymal Stem Cells and the Effects on Global Gene Expression.Stem cells international · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vitrification is commonly used in the cryopreservation of mammalian blastocysts to overcome the temporal and spatial limitations of embryo transfer. Previous studies have shown that the implantation ability of vitrified blastocysts is impaired and that microRNAs (miRNAs) regulate the critical genes for embryo implantation. However, little information is available about the effect of vitrification on the miRNA transcriptome in blastocysts. In the present study, the miRNA transcriptomes in fresh and vitrified mouse blastocysts were analyzed by miRNA Taqman assay based method, and the results were validated using quantitative real-time PCR (qRT-PCR). Then, the differentially expressed miRNAs were assessed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Overall, 760 known mouse miRNAs were detected in the vitrified and fresh mouse blastocysts. Of these, the expression levels of five miRNAs differed significantly: in the vitrified blastocysts, four miRNAs (mmu-miR-199a-5p, mmu-miR-329-3p, mmu-miR-136-5p and mmu-miR-16-1-3p) were upregulated, and one (mmu-miR-212-3p) was downregulated. The expression levels of all miRNAs measured by the miRNA Taqman assay based method and qRT-PCR were consistent. The four upregulated miRNAs were predicted to regulate 877 candidate target genes, and the downregulated miRNA was predicted to regulate 231 genes. The biological analysis further showed that the differentially expressed miRNAs mainly regulated the implantation of embryos. In conclusion, the results of our study showed that vitrification significantly altered the miRNA transcriptome in mouse blastocysts, which may decrease the implantation potential of vitrified blastocysts.
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