ArticlePloS one2015
Dose-dependent ATP depletion and cancer cell death following calcium electroporation, relative effect of calcium concentration and electric field strength.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. It is linked to trial NCT03051269 (Calcium Electroporation for Head and Neck Cancer), which is not on this map. Cited by 54 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Calcium Electroporation for Head and Neck Cancer
Who cites it
54 citing papers in PubMed, 94 citations in OpenAlex.
- Clinical Advantages and Pitfalls of Pulsed Field Ablation: How to Prevent ALH Syndrome.Journal of arrhythmia · 2026Review
- Thermal preconditioning of targeted tissue enhances pulsed field ablation lesions.Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing · 2026Article
- Evaluation of unipolar and bipolar nanosecond pulses for calcium electrochemotherapy and immune response.Frontiers in immunology · 2026Article
- Effects of ultra-fast nanosecond electric pulses on mitochondria transmembrane potential and oxidation.Scientific reports · 2025Article
- CaUltrasonics sonochemistry · 2025Article
- Calcium Electrochemotherapy and Challenges in Combined Treatment with Dendritic Cell Vaccination.Pharmaceutics · 2025Article
- Pulsed Field Ablation: A Review of Preclinical and Clinical Studies.Bioengineering (Basel, Switzerland) · 2025Review
- Power-Driven Electroporation Is Predictive of Treatment Outcomes in a Conductivity-Independent Manner.BME frontiers · 2025Article
- Electroporation with Calcium or Bleomycin: First Application in an In Vivo Uveal Melanoma Patient-Derived Xenograft Model.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Recent Insights into Nanotechnology in Colorectal Cancer.Applied biochemistry and biotechnology · 2024Review
- Immune cell populations in the tumour environment following calcium electropora-tion for cutaneous metastasis: a histopathological study.Acta oncologica (Stockholm, Sweden) · 2024Article
- Utilizing Human-Induced Pluripotent Stem Cells to Study Cardiac Electroporation Pulsed-Field Ablation.Circulation. Arrhythmia and electrophysiology · 2024Article
- Electrolysis products, reactive oxygen species and ATP loss contribute to cell death following irreversible electroporation with microsecond-long pulsed electric fields.Bioelectrochemistry (Amsterdam, Netherlands) · 2024Article
- Dynamics of cell membrane lesions and adaptive conductance under the electrical stress.Cell stress · 2024Article
- Determination of the Impact of High-Intensity Pulsed Electromagnetic Fields on the Release of Damage-Associated Molecular Pattern Molecules.International journal of molecular sciences · 2023Article
- Calcium Electrochemotherapy for Tumor Eradication and the Potential of High-Frequency Nanosecond Protocols.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Acute ATP loss during irreversible electroporation mediates caspase independent cell death.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- The effectiveness of calcium electroporation combined with gene electrotransfer of a plasmid encoding IL-12 is tumor type-dependent.Frontiers in immunology · 2023Article
- Pulsed-field ablation: Computational modeling of electric fields for lesion depth analysis.Heart rhythm O2 · 2022Article
- Calcium Electroporation Reduces Viability and Proliferation Capacity of Four Uveal Melanoma Cell Lines in 2D and 3D Cultures.Cancers · 2022Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundElectroporation, a method for increasing the permeability of membranes to ions and small molecules, is used in the clinic with chemotherapeutic drugs for cancer treatment (electrochemotherapy). Electroporation with calcium causes ATP (adenosine triphosphate) depletion and cancer cell death and could be a novel cancer treatment. This study aims at understanding the relationship between applied electric field, calcium concentration, ATP depletion and efficacy.
methodsIn three human cell lines--H69 (small-cell lung cancer), SW780 (bladder cancer), and U937 (leukaemia), viability was determined after treatment with 1, 3, or 5 mM calcium and eight 99 μs pulses with 0.8, 1.0, 1.2, 1.4 or 1.6 kV/cm. Fitting analysis was applied to quantify the cell-killing efficacy in presence of calcium. Post-treatment intracellular ATP was measured in H69 and SW780 cells. Post-treatment intracellular ATP was observed with fluorescence confocal microscopy of quinacrine-labelled U937 cells.
resultsBoth H69 and SW780 cells showed dose-dependent (calcium concentration and electric field) decrease in intracellular ATP (p<0.05) and reduced viability. The 50% effective cell kill was found at 3.71 kV/cm (H69) and 3.28 kV/cm (SW780), reduced to 1.40 and 1.15 kV/cm (respectively) with 1 mM calcium (lower EC50 for higher calcium concentrations). Quinacrine fluorescence intensity of calcium-electroporated U937 cells was one third lower than in controls (p<0.0001).
conclusionsCalcium electroporation dose-dependently reduced cell survival and intracellular ATP. Increasing extracellular calcium allows the use of a lower electric field. GENERAL SIGNIFICANCE: This study supports the use of calcium electroporation for treatment of cancer and possibly lowering the applied electric field in future trials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.