ArticlePloS one2015
Therapeutic effects of topical netrin-4 inhibits corneal neovascularization in alkali-burn rats.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- Therapeutic potential of Jatimukuladi Varti Anjana in alkali burn-induced corneal injury and neovascularization in albino rats.Frontiers in medicine · 2026Article
- Netrin-4 Modulates Corneal Wound Healing by Enhancing Corneal Epithelium and Nerve Regeneration.FASEB bioAdvances · 2025Article
- Elevated Netrin-4 Expression and Its Action in Infrapatellar Fat Pad.International journal of molecular sciences · 2024Article
- RNA‑binding protein quaking 5 inhibits the progression of non‑small cell lung cancer by upregulating netrin‑4 expression.Oncology reports · 2023Article
- Netrin-4: Focus on Its Role in Axon Guidance, Tissue Stability, Angiogenesis and Tumors.Cellular and molecular neurobiology · 2023Review
- Pathogenesis of Alkali Injury-Induced Limbal Stem Cell Deficiency: A Literature Survey of Animal Models.Cells · 2023Review
- Article
- Expression of axon guidance ligands and their receptors in the cornea and trigeminal ganglia and their recovery after corneal epithelium injury.Experimental eye research · 2022Article
- Netrin-1 promotes epithelium repair in corneal injury.International journal of ophthalmology · 2020Article
- Targeting Netrin-1 and -4 as a Novel Diagnostic Parameter and Treatment Option for Diabetic Retinopathy.Clinical ophthalmology (Auckland, N.Z.) · 2020Review
- Article
- Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration.International journal of molecular sciences · 2019Article
- Netrin Family: Role for Protein Isoforms in Cancer.Journal of nucleic acids · 2019Review
- Evidence of the involvement of dystrophin Dp71 in corneal angiogenesis.Molecular vision · 2019Article
- EGF/EGFR upregulates and cooperates with Netrin-4 to protect glioblastoma cells from DNA damage-induced senescence.BMC cancer · 2018Article
- Current and emerging therapies for corneal neovascularization.The ocular surface · 2018Review
- Differential Distribution of Laminin N-Terminus α31 Across the Ocular Surface: Implications for Corneal Wound Repair.Investigative ophthalmology & visual science · 2018Article
- Antiangiogenic effects of catalpol on rat corneal neovascularization.Molecular medicine reports · 2018Article
- Structural decoding of netrin-4 reveals a regulatory function towards mature basement membranes.Nature communications · 2016Article
- Effects of intravitreal injection of netrin-1 in retinal neovascularization of streptozotocin-induced diabetic rats.Drug design, development and therapy · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Netrins are secreted molecules involved in axon guidance and angiogenesis. However, the role of netrins in the vasculature remains unclear. Netrin-4 and netrin-1 have been found to be either pro- or antiangiogenic factors. Previously, we found that netrin-1 acts as an anti-angiogenic factor in rats by inhibiting alkali burn-induced corneal neovascularization. Here, we further investigate the effects of netrin-4, another member of the same netrin family, on neovascularization in vitro and in vivo. We found that netrin-4 functions similarly as netrin-1 in angiogenesis. In vitro angiogenesis assay shows that netrin-4 affected human umbilical vein endothelial cell (HUVEC) tube formation, viability and proliferation, apoptosis, migration, and invasion in a dose-dependent manner. Netrin-4 was topically applied in vivo to alkali-burned rat corneas on day 0 (immediately after injury) and/or day 10 post-injury. Netrin-4 subsequently suppressed and reversed corneal neovascularization. Netrin-4 inhibited corneal epithelial and stromal cell apoptosis, inhibited vascular endothelial growth factor (VEGF), but promoted pigment epithelium-derived factor (PEDF) expression, decreased NK-KB p65 expression, and inhibits neutrophil and macrophage infiltration. These results indicate that netrin-4 shed new light on its potential roles in treatmenting for angiogenic diseases that affect the ocular surface, as well as other tissues.
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