Evidence map›Paper›PMID 25853484›Full record

ArticleViruses2015

A suggested new bacteriophage genus, "Kp34likevirus", within the Autographivirinae subfamily of Podoviridae.

Harald Eriksson, Barbara Maciejewska, Agnieszka Latka, Grazyna Majkowska-Skrobek, Marios Hellstrand, Öjar Melefors, Jin-Town Wang, Andrew M Kropinski, Zuzanna Drulis-Kawa, Anders S Nilsson

Abstract read
In one paragraph

Article in Viruses, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  16. Isolation and Characterization of TwoInternational journal of molecular sciences · 2020
    Article
  17. Article
  18. Bacteriophages ofJournal of medical microbiology · 2020
    Review
  19. Isolation of Four Lytic Phages InfectingInternational journal of molecular sciences · 2020
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Harald ErikssonDepartment of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Stockholm 106 91, Sweden. harald.eriksson@su.se.
Barbara MaciejewskaInstitute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland. barbara-boczkowska@wp.pl.
Agnieszka LatkaInstitute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland. agnieszkalatka1989@gmail.com.
Grazyna Majkowska-SkrobekInstitute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland.
Marios HellstrandDepartment of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Stockholm 106 91, Sweden. marios.hellstrand@gmail.com.
Öjar MeleforsDepartment of Microbiology, Public Health Agency of Sweden, Solna 171 82, Sweden. ojar.melefors@ki.se.
Jin-Town WangDepartment of Microbiology, National Taiwan University, College of Medicine, Taipei 10051, Taiwan. wangjt@ntu.edu.tw.
Andrew M KropinskiDepartments of Food Science, Molecular & Cellular Biology, and Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada. kropinsk@queensu.ca.
Zuzanna Drulis-KawaInstitute of Genetics and Microbiology, University of Wroclaw, Wroclaw 51-148, Poland.
Anders S NilssonDepartment of Molecular Biosciences, the Wenner-Gren Institute, Stockholm University, Stockholm 106 91, Sweden. anders.s.nilsson@su.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Klebsiella pneumoniae phages vB_KpnP_SU503 (SU503) and vB_KpnP_SU552A (SU552A) are virulent viruses belonging to the Autographivirinae subfamily of Podoviridae that infect and kill multi-resistant K. pneumoniae isolates. Phages SU503 and SU552A show high pairwise nucleotide identity to Klebsiella phages KP34 (NC_013649), F19 (NC_023567) and NTUH-K2044-K1-1 (NC_025418). Bioinformatic analysis of these phage genomes show high conservation of gene arrangement and gene content, conserved catalytically active residues of their RNA polymerase, a common and specific lysis cassette, and form a joint cluster in phylogenetic analysis of their conserved genes. Also, we have performed biological characterization of the burst size, latent period, host specificity (together with KP34 and NTUH-K2044-K1-1), morphology, and structural genes as well as sensitivity testing to various conditions. Based on the analyses of these phages, the creation of a new phage genus is suggested within the Autographivirinae, called "Kp34likevirus" after their type phage, KP34. This genus should encompass the recently genome sequenced Klebsiella phages KP34, SU503, SU552A, F19 and NTUH-K2044-K1-1.

Indexed as

BacteriophagesComputational BiologyElectrophoresis, Polyacrylamide GelGene OrderGenes, ViralHost SpecificityKlebsiellaMicroscopy, Electron, TransmissionPodoviridaeSyntenyViral Structural ProteinsVirionViral Structural Proteins

Identifiers

PMID25853484
PMCPMC4411677

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.