ArticleCurrent chemical genomics and translational medicine2015
A facile method for simultaneously measuring neuronal cell viability and neurite outgrowth.
Article in Current chemical genomics and translational medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
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- Extracellular Hsp90α stimulates a unique innate gene profile in microglial cells with simultaneous activation of Nrf2 and protection from oxidative stress.Cell stress & chaperones · 2022Article
- Optimised techniques for high-throughput screening of differentiated SH-SY5Y cells and application for neurite outgrowth assays.Scientific reports · 2021Article
- Caspase inhibition rescues F1Fo ATP synthase dysfunction-mediated dendritic spine elimination.Scientific reports · 2020Article
- The Roles of an Aluminum Underlayer in the Biocompatibility and Mechanical Integrity of Vertically Aligned Carbon Nanotubes for Interfacing with Retinal Neurons.Micromachines · 2020Article
- Caffeine and NADAntioxidants (Basel, Switzerland) · 2020Article
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- Using PROMIS® to create clinically meaningful profiles of nephrotic syndrome patients.Health psychology : official journal of the Division of Health Psychology, American Psychological Association · 2019Article
- Trkb-IP3 Pathway Mediating Neuroprotection in Rat Hippocampal Neuronal Cell Culture Following Induction of Kainic Acid.The Malaysian journal of medical sciences : MJMS · 2018Article
- Scalable Production of iPSC-Derived Human Neurons to Identify Tau-Lowering Compounds by High-Content Screening.Stem cell reports · 2017Article
- Exercise and BDNF reduce Aβ production by enhancing α-secretase processing of APP.Journal of neurochemistry · 2017Article
- Quantitative assessment of neural outgrowth using spatial light interference microscopy.Journal of biomedical optics · 2017Article
- In vitro models of axon regeneration.Experimental neurology · 2017Review
- Analysis of Gene Expression and Neuronal Phenotype in Neuroscreen-1 (NS-1) Cells.International journal of biomedical investigationArticle
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurite outgrowth is an important morphological phenotype of neuronal cells that correlates with their function and cell health, yet there are limited methods available for measuring this phenomenon. Current approaches to measuring neurite outgrowth are laborious and time-consuming, relying largely upon immunocytochemical staining of neuronal markers (e.g., beta-III tubulin or MAP2) followed by manual or automated microscopy for image acquisition and analysis. Here we report the development of a quick and simple dual-color fluorescent dye-based staining method that allows for the simultaneous measurement of neuronal cell health and relative neurite outgrowth from the same sample. An orangered fluorescent dye that stains cell membrane surfaces is used as an indirect reporter of changes in relative neurite outgrowth due to alterations in the number or length of membrane projections emanating from neuronal cell bodies. Cell viability is assessed simultaneously via the use of a cell-permeant dye that is converted by intracellular esterase activity from a non-fluorescent substrate to a green-fluorescent product. Using Neuroscreen-1 cells (a PC-12 subclone), primary rat cortex neurons, and human induced pluripotent stem cell (iPSC)-derived neurons, we demonstrate that this multiplex assay allows for rapid visualization and unbiased, quantitative plate reader analysis of neuronal cell health and neurite outgrowth.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.