ArticleAMB Express2015
Enhanced xylose fermentation and ethanol production by engineered Saccharomyces cerevisiae strain.
Article in AMB Express, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Insights into cell robustness against lignocellulosic inhibitors and insoluble solids in bioethanol production processes.Scientific reports · 2022Article
- Rational engineering of Saccharomyces cerevisiae towards improved tolerance to multiple inhibitors in lignocellulose fermentations.Biotechnology for biofuels · 2021Article
- Current Status and Applications of Adaptive Laboratory Evolution in Industrial Microorganisms.Journal of microbiology and biotechnology · 2020Review
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Understanding xylose isomerase from Burkholderia cenocepacia: insights into structure and functionality for ethanol production.AMB Express · 2019Article
- Rewired cellular signaling coordinates sugar and hypoxic responses for anaerobic xylose fermentation in yeast.PLoS genetics · 2019Article
- Engineering of Pentose Transport inFrontiers in bioengineering and biotechnology · 2019Review
- Molecular evolutionary engineering of xylose isomerase to improve its catalytic activity and performance of micro-aerobic glucose/xylose co-fermentation inBiotechnology for biofuels · 2019Article
- Article
- xylA and xylB overexpression as a successful strategy for improving xylose utilization and poly-3-hydroxybutyrate production in Burkholderia sacchari.Journal of industrial microbiology & biotechnology · 2018Article
- Augmenting Pentose Utilization and Ethanol Production of NativeFrontiers in bioengineering and biotechnology · 2018Article
- The amino-terminal tail of Hxt11 confers membrane stability to the Hxt2 sugar transporter and improves xylose fermentation in the presence of acetic acid.Biotechnology and bioengineering · 2017Article
- The PWWP domain of the human oncogene WHSC1L1/NSD3 induces a metabolic shift toward fermentation.Oncotarget · 2017Article
- Xylose Fermentation by Saccharomyces cerevisiae: Challenges and Prospects.International journal of molecular sciences · 2016Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently demonstrated that heterologous expression of a bacterial xylose isomerase gene (xylA) of Burkholderia cenocepacia enabled a laboratorial Saccharomyces cerevisiae strain to ferment xylose anaerobically, without xylitol accumulation. However, the recombinant yeast fermented xylose slowly. In this study, an evolutionary engineering strategy was applied to improve xylose fermentation by the xylA-expressing yeast strain, which involved sequential batch cultivation on xylose. The resulting yeast strain co-fermented glucose and xylose rapidly and almost simultaneously, exhibiting improved ethanol production and productivity. It was also observed that when cells were grown in a medium containing higher glucose concentrations before being transferred to fermentation medium, higher rates of xylose consumption and ethanol production were obtained, demonstrating that xylose utilization was not regulated by catabolic repression. Results obtained by qPCR demonstrate that the efficiency in xylose fermentation showed by the evolved strain is associated, to the increase in the expression of genes HXT2 and TAL1, which code for a low-affinity hexose transporter and transaldolase, respectively. The ethanol productivity obtained after the introduction of only one genetic modification and the submission to a one-stage process of evolutionary engineering was equivalent to those of strains submitted to extensive metabolic and evolutionary engineering, providing solid basis for future applications of this strategy in industrial strains.
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